Enfer Scientific, Naas, County Kildare, Ireland.
Synergy Farm Health, Maiden Newton, Dorset, United Kingdom.
PLoS One. 2024 Apr 30;19(4):e0301609. doi: 10.1371/journal.pone.0301609. eCollection 2024.
Bovine tuberculosis is usually diagnosed using tuberculin skin tests or at post-mortem. Recently, we have developed a serological test for bovine tuberculosis in cattle which shows a high degree of accuracy using serum samples. Here, we have assessed the performance of the test using individual bovine milk samples. The diagnostic specificity estimate using the high sensitivity setting of the test was 99.7% (95% CI: 99.2-99.9). This estimate was not altered significantly by tuberculin boosting. The relative sensitivity estimates of the test using the high sensitivity setting in milk samples from comparative skin test positive animals was 90.8% (95% CI: 87.1-93.6) with boosting. In animals with lesions, the relative sensitivity was 96.0% (95% CI: 89.6-98.7). Analysis of paired serum and milk samples from skin test positive animals showed correlation coefficients ranging from 0.756-0.955 for individual antigens used in the test. Kappa analysis indicated almost perfect agreement between serum and milk results, while McNemar marginal homogeneity analysis showed no statistically significant differences between the two media. The positive and negative likelihood ratio were 347.8 (95% CI: 112.3-1077.5) and 0.092 (95% CI: 0.07-0.13) respectively for boosted samples from skin test positive animals. The results show that the test has high sensitivity and specificity in individual milk samples and thus milk samples could be used for the diagnosis of bovine tuberculosis.
牛结核病通常使用结核菌素皮肤试验或死后进行诊断。最近，我们开发了一种用于牛结核病的血清学检测方法，该方法使用血清样本具有很高的准确性。在这里，我们使用个体牛牛奶样本评估了该检测的性能。使用该检测的高灵敏度设置的诊断特异性估计值为 99.7%（95%CI：99.2-99.9）。该估计值不受结核菌素增强的显著影响。使用高灵敏度设置在比较皮肤试验阳性动物的牛奶样本中，该检测的相对灵敏度估计值为 90.8%（95%CI：87.1-93.6），增强后为 96.0%（95%CI：89.6-98.7）。在有病变的动物中，相对灵敏度为 96.0%（95%CI：89.6-98.7）。分析来自皮肤试验阳性动物的配对血清和牛奶样本显示，用于该检测的单个抗原的相关系数范围为 0.756-0.955。Kappa 分析表明血清和牛奶结果之间几乎完全一致，而 McNemar 边缘同质性分析表明两种介质之间没有统计学上的显著差异。来自皮肤试验阳性动物的增强样本的阳性和阴性似然比分别为 347.8（95%CI：112.3-1077.5）和 0.092（95%CI：0.07-0.13）。结果表明，该检测在个体牛奶样本中具有很高的敏感性和特异性，因此牛奶样本可用于牛结核病的诊断。
