McFarland E W, Neuringer L J, Kushmerick M J
Francis Bitter National Magnet Laboratory, Massachusetts Institute of Technology, Cambridge 02139.
Magn Reson Imaging. 1988 Sep-Oct;6(5):507-15. doi: 10.1016/0730-725x(88)90125-7.
Systems investigated with NMR spectroscopy are sometimes heterogeneous with respect to chemical composition, rates of chemical exchange, and other properties influencing magnetic resonance parameters. A method was developed to spatially encode reaction kinetic information and produce NMR images sensitive to chemical exchange. A modified spin-echo pulse sequence was used to allow chemical shift-selective imaging and chemical exchange encoding. 1H and 31P images with microscopic resolution were obtained which yielded chemical exchange as a function of position. Chemical exchange images of the base-catalyzed proton exchange of acetylacetone and of the enzyme-catalyzed 31P transfer between PCr and ATP were obtained at 8.4 T in phantoms at 360 and 146 MHz, respectively. These images demonstrate a means of investigating kinetic heterogeneity and compartmentalization of reactions that are important in the study of both living and non-living systems.
利用核磁共振光谱研究的体系在化学成分、化学交换速率以及影响磁共振参数的其他性质方面有时是不均匀的。已开发出一种方法来对反应动力学信息进行空间编码，并生成对化学交换敏感的核磁共振图像。使用了一种改进的自旋回波脉冲序列来实现化学位移选择性成像和化学交换编码。获得了具有微观分辨率的氢质子和磷-31图像，这些图像给出了作为位置函数的化学交换情况。分别在360兆赫兹和146兆赫兹下，于体模中在8.4特斯拉的磁场强度下获得了乙酰丙酮碱催化质子交换以及磷酸肌酸和三磷酸腺苷之间酶催化的磷-31转移的化学交换图像。这些图像展示了一种研究反应动力学不均匀性和反应区室化的手段，这对于生物和非生物体系的研究都很重要。
