Sankar S S, Molé P A, Coulson R L
Magn Reson Med. 1986 Dec;3(6):958-62. doi: 10.1002/mrm.1910030618.
1H NMR spin-lattice relaxation times (T1) of the N-CH3 proton resonances of phosphocreatine (PCr) and creatine (Cr) in water solutions were obtained using the 1,3,3,1 pulse sequence. These T1 values were equivalent to those obtained in D2O and water using either the conventional inversion-recovery experiment or the 1,3,3,1 pulse sequence. Thus, the 1,3,3,1 sequence of proton NMR can provide an independent means along with phosphorous NMR for assess PCr and for the study of the creatine kinase reaction (PCr + ADP in equilibrium ATP + Cr) in aqueous solutions and perhaps in biological preparations.
使用1,3,3,1脉冲序列获得了水溶液中磷酸肌酸（PCr）和肌酸（Cr）的N-CH3质子共振的1H NMR自旋晶格弛豫时间（T1）。这些T1值与使用传统反转恢复实验或1,3,3,1脉冲序列在D2O和水中获得的值相当。因此，质子NMR的1,3,3,1序列可以与磷NMR一起提供一种独立的方法，用于评估PCr以及研究水溶液中或许还有生物制剂中的肌酸激酶反应（PCr + ADP处于平衡ATP + Cr状态）。
