Xiao Ying, Hu Zhongzhi, Yin Zhiting, Zhou Yiming, Liu Taiyi, Zhou Xiaoli, Chang Dawei
School of Perfume and Aroma Technology, Shanghai Institute of TechnologyShanghai, China.
School of Food and Biological Engineering, Shaanxi University of Science and TechnologyXi'an, China.
Front Pharmacol. 2017 May 4;8:231. doi: 10.3389/fphar.2017.00231. eCollection 2017.
The metabolite profiles and distributions of procyanidin B2 were qualitatively described using UPLC-DAD-ESI-IT-TOF-MS without help of reference standards, and a possible metabolic pathway was proposed in the present study. Summarily, 53 metabolites (24 new metabolites) were detected as metabolites of procyanidin B2, and 45 of them were tentatively identified. Twenty seven metabolites were assigned as similar metabolites of (-)-epicatechin by scission of the flavanol interflavanic bond C4-C8, including 16 aromatic metabolites, 5 conjugated metabolites, 3 ring-cleavage metabolites, and 2 phenylvalerolactone metabolites. Additionally, 14 metabolites were conjugates of free procyanidin B2, comprising 9 methylation metabolites, 8 sulfation metabolites, 5 hydration metabolites, 2 hydroxylation metabolites, 1 hydrogenation metabolites, and 1 glucuronidation metabolites. The results of metabolite distributions in organs indicated that the conjugated reaction of free procyanidin B2 mainly occurred in liver and diversified metabolites forms were observed in small intestine. The metabolic components of procyanidin B2 identified in mice provided useful information for further study of the bioactivity and mechanism of its action.
在没有参考标准品帮助的情况下，使用超高效液相色谱-二极管阵列-电喷雾电离-离子阱-飞行时间质谱（UPLC-DAD-ESI-IT-TOF-MS）对原花青素B2的代谢产物谱及其分布进行了定性描述，并在本研究中提出了一条可能的代谢途径。总之，共检测到53种代谢产物（24种新代谢产物）为原花青素B2的代谢产物，其中45种被初步鉴定。通过断裂黄烷醇间的C4-C8键，有27种代谢产物被归类为（-）-表儿茶素的类似代谢产物，包括16种芳香族代谢产物、5种共轭代谢产物、3种环裂解代谢产物和2种苯基戊内酯代谢产物。此外，有14种代谢产物是游离原花青素B2的共轭物，包括9种甲基化代谢产物、8种硫酸化代谢产物、5种水合代谢产物、2种羟基化代谢产物、1种氢化代谢产物和1种葡萄糖醛酸化代谢产物。各器官中代谢产物分布的结果表明，游离原花青素B2的共轭反应主要发生在肝脏，在小肠中观察到多种代谢产物形式。在小鼠体内鉴定出的原花青素B2的代谢成分，为进一步研究其生物活性及作用机制提供了有用信息。
