Kawai N, Saito M, Ohsako S
Department of Neurobiology, Tokyo Metropolitan Institute for Neurosciences, Japan.
Neurosci Lett. 1988 Dec 19;95(1-3):203-7. doi: 10.1016/0304-3940(88)90657-x.
In the neuromuscular synapse of lobster walking leg, application of glutamate and its agonists generated transient depolarization of the postsynaptic membrane. The order of potency was quisqualate greater than glutamate greater than kainate. NMDA was inactive on lobster muscle. However, the membrane of Xenopus oocytes injected with mRNA from lobster muscle acquired sensitivity to NMDA. The order of potency for inducing current responses in the oocyte membrane was kainate greater than glutamate = NMDA much greater than quisqualate. The results suggest that mRNAs coding for glutamate receptors in the lobster muscle are expressed differently in Xenopus oocyte membrane.
在龙虾步行腿的神经肌肉突触中，应用谷氨酸及其激动剂会使突触后膜产生短暂去极化。效力顺序为喹啉酸大于谷氨酸大于海人酸。NMDA对龙虾肌肉无活性。然而，注射了龙虾肌肉mRNA的非洲爪蟾卵母细胞膜对NMDA产生了敏感性。在卵母细胞膜中诱导电流反应的效力顺序为海人酸大于谷氨酸 = NMDA远大于喹啉酸。结果表明，编码龙虾肌肉中谷氨酸受体的mRNA在非洲爪蟾卵母细胞膜中的表达有所不同。
