Solanki Amit Kumar, Bhatia Bharati, Kaushik Himani, Deshmukh Sachin K, Dixit Aparna, Garg Lalit C
Gene Regulation Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
Gene Regulation Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi, 110067, India.
Appl Microbiol Biotechnol. 2017 Jul;101(14):5699-5708. doi: 10.1007/s00253-017-8333-2. Epub 2017 May 18.
Clostridium perfringens beta toxin (CPB) is the primary pathogenic factor responsible for necrotic enteritis in sheep, cattle and humans. Owing to rapid progression of the disease, vaccination is the only possible recourse to avoid high mortality in animal farms and huge economic losses. The present study reports evaluation of a cpb gene-based DNA vaccine encoding the beta toxin of C. perfringens with homologous as well as heterologous booster strategy. Immunization strategy employing heterologous booster with heat-inactivated rCPB mounted stronger immune response when compared to that generated by homologous booster. Antibody isotyping and cytokine ELISA demonstrated the immune response to be Th1-biased mixed immune response. While moderate protection of immunized BALB/c and C57BL/6 mice against rCPB challenge was observed with homologous booster strategy, heterologous booster strategy led to complete protection. Thus, beta toxin-based DNA vaccine using the heterologous prime-boosting strategy was able to generate better immune response and conferred greater degree of protection against high of dose rCPB challenge than homologous booster regimen, making it an effective vaccination approach against C. perfringens beta toxin.
产气荚膜梭菌β毒素（CPB）是导致绵羊、牛和人类坏死性肠炎的主要致病因素。由于该疾病进展迅速，接种疫苗是避免养殖场高死亡率和巨大经济损失的唯一可行办法。本研究报告了对一种基于cpb基因的DNA疫苗的评估，该疫苗采用同源和异源加强策略编码产气荚膜梭菌的β毒素。与同源加强相比，采用热灭活rCPB进行异源加强的免疫策略产生了更强的免疫反应。抗体分型和细胞因子ELISA表明免疫反应是以Th1为主的混合免疫反应。虽然同源加强策略对免疫的BALB/c和C57BL/6小鼠抵抗rCPB攻击有适度保护作用，但异源加强策略导致了完全保护。因此，与同源加强方案相比，使用异源初免-加强策略的基于β毒素的DNA疫苗能够产生更好的免疫反应，并对高剂量rCPB攻击提供更大程度的保护，使其成为一种针对产气荚膜梭菌β毒素的有效疫苗接种方法。
