Anderson David M G, Lambert Wendi, Calkins David J, Ablonczy Zsolt, Crouch Rosalie K, Caprioli Richard M, Schey Kevin L
Mass Spectrometry Research Center, Department of Biochemistry, Vanderbilt University School of Medicine, 9126D MRB III, 465 21st Ave., South, Nashville, TN, 37232, USA.
Department of Ophthalmology and Visual Sciences, Vanderbilt University Medical Center, Nashville, TN, USA.
Methods Mol Biol. 2017;1618:15-27. doi: 10.1007/978-1-4939-7051-3_3.
The visual system is comprised of many specialized cell types that are essential for relaying sensory information about an animal's surroundings to the brain. The cells present in ocular tissue are notoriously delicate, making it particularly challenging to section thin slices of unfixed tissue. Maintaining the morphology of the native tissue is crucial for accurate observations by either conventional staining techniques or in this instance matrix-assisted laser desorption ionization (MALDI IMS) or imaging using mass spectrometry. As vision loss is a significantly debilitating condition, studying molecular mechanisms involved in the process of vision loss is a critically important area of research.
视觉系统由许多特殊的细胞类型组成，这些细胞类型对于将有关动物周围环境的感官信息传递到大脑至关重要。眼部组织中的细胞非常脆弱，这使得切割未固定组织的薄片特别具有挑战性。对于通过传统染色技术进行准确观察，或者在此实例中通过基质辅助激光解吸电离（MALDI IMS）或质谱成像来维持天然组织的形态至关重要。由于视力丧失是一种严重使人衰弱的病症，研究视力丧失过程中涉及的分子机制是一个极其重要的研究领域。
