Sata T, Linden J, Liu L W, Kubota E, Said S I
Department of Medicine, University of Illinois College of Medicine, Chicago 60612.
Peptides. 1988 Jul-Aug;9(4):853-8. doi: 10.1016/0196-9781(88)90133-7.
We have investigated VIP-induced relaxation and cyclic AMP accumulation in rat thoracic aorta strips, and the importance of endothelium to both actions. The relaxation was greatly attenuated by removal of endothelium, but was unaltered by cyclo-oxygenase or lipoxygenase inhibitors. Similarly, cyclic AMP formation was nearly abolished with loss of endothelium, but was largely unaffected by inhibitors of arachidonate pathways, cytochrome P450 or guanylate cyclase. VIP may stimulate the release of a diffusible factor from endothelium (an EDRF), which activates adenylate cyclase and relaxes aortic smooth muscle.
我们研究了血管活性肠肽（VIP）诱导的大鼠胸主动脉条舒张以及环磷酸腺苷（cAMP）的积累，以及内皮对这两种作用的重要性。去除内皮后，舒张作用大大减弱，但环氧化酶或脂氧化酶抑制剂对其无影响。同样，内皮缺失时cAMP的形成几乎被消除，但花生四烯酸途径、细胞色素P450或鸟苷酸环化酶的抑制剂对其影响不大。VIP可能刺激内皮释放一种可扩散因子（一种内皮舒张因子），该因子激活腺苷酸环化酶并使主动脉平滑肌舒张。
