Sivaramakrishnan Ramachandran, Incharoensakdi Aran
Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
J Phycol. 2017 Aug;53(4):855-868. doi: 10.1111/jpy.12549. Epub 2017 Jul 3.
The biochemical contents and biodiesel production ability of three microalgal strains grown under different sodium nitrate, sodium carbonate, and ferric ammonium citrate (iron) levels were investigated. The highest biomass and lipid contents were found in Scenedesmus sp., Chlorella sp., and Chlamydomonas sp. when grown in normal BG-11 containing sodium carbonate concentration at 0.03 g · L , and in normal BG-11 containing iron concentration (IC) at 0.009 or 0.012 g · L . Increasing the sodium nitrate level increased the biomass content, but decreased the lipid content in all three microalgae. Among the three microalgae, Scenedesmus sp. showed the highest total lipid yield of 0.69 g · L under the IC of 0.012 g · L . Palmitic and oleic acids were the major fatty acids of Scenedesmus sp. and Chlamydomonas sp. lipids. On the other hand, Chlorella sp. lipids were rich in palmitic, oleic, and linolenic acids, and henceforth contributing to poor biodiesel properties below the standard limits. The three isolated strains had a potential for biodiesel production. Nevertheless, Scenedesmus sp. from stone quarry pond water was the most suitable source for biodiesel production with tolerance toward the high concentration of sodium carbonate without the loss of its biodiesel properties.
研究了在不同硝酸钠、碳酸钠和柠檬酸铁铵（铁）水平下生长的三种微藻菌株的生化成分和生物柴油生产能力。当在含有浓度为0.03 g·L的碳酸钠的正常BG-11中，以及在含有铁浓度（IC）为0.009或0.012 g·L的正常BG-11中生长时，在栅藻属、小球藻属和衣藻属中发现了最高的生物量和脂质含量。提高硝酸钠水平会增加所有三种微藻的生物量含量，但会降低脂质含量。在这三种微藻中，在IC为0.012 g·L时，栅藻属的总脂质产量最高，为0.69 g·L。棕榈酸和油酸是栅藻属和衣藻属脂质的主要脂肪酸。另一方面，小球藻属的脂质富含棕榈酸、油酸和亚麻酸，因此导致生物柴油性能低于标准限值。这三种分离菌株具有生物柴油生产潜力。然而，来自采石场池塘水的栅藻属是最适合生物柴油生产的来源，它对高浓度碳酸钠具有耐受性，且不会损失其生物柴油性能。
