Environmental Biotechnology laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, 793022, India.
Environ Sci Pollut Res Int. 2019 Jul;26(20):20815-20828. doi: 10.1007/s11356-019-05065-6. Epub 2019 May 20.
The effects of iron (Fe), zinc (Zn), and molybdenum (Mo) on the biomass yield, lipid content, lipid yield, and fatty acid composition of Chlorella sp. NC-MKM, Graesiella emersonii NC-M1, Scenedesmus acutus NC-M2, and Chlorophyta sp. NC-M5 were studied. Among them, G. emersonii NC-M1 recorded the highest percentage increase in lipid content (140.3%) and neutral lipid (50.9%) under Zn-supplemented condition compared to the control. Also, it showed a 105% and 41.88% increase in lipid yield and neutral lipid under Fe-supplemented condition compared to the control. However, Chlorella sp. NC-MKM recorded an elevation in lipid yield (70.3% rise) and neutral lipid (24.32% rise) compared to the control in Mo-supplemented condition. The enhanced production of reactive oxygen species (ROS) and antioxidant enzyme (SOD and POD) under Fe-, Zn-, and Mo-supplemented condition supports the lipid accumulation. FAME analysis showed that the overall percentage of SFA and MUFA increased after the addition of Fe, Zn, and Mo in a culture medium compared to the control which is vital for a good-quality biodiesel. Further, biodiesel properties derived from FAMEs such as CN, SV, IV, CFPP, OS, υ, ρ, and HHV were found in accordance with biodiesel standard.
研究了铁（Fe）、锌（Zn）和钼（Mo）对小球藻 sp. NC-MKM、格氏海链藻 NC-M1、普通小球藻 NC-M2 和绿藻 sp. NC-M5 的生物量产量、脂质含量、脂质产量和脂肪酸组成的影响。其中，与对照相比，在添加 Zn 的条件下，G. emersonii NC-M1 的脂质含量（增加 140.3%）和中性脂质（增加 50.9%）的百分比增幅最高。此外，与对照相比，在添加 Fe 的条件下，其脂质产量和中性脂质分别增加了 105%和 41.88%。然而，在添加 Mo 的条件下，小球藻 sp. NC-MKM 的脂质产量（增加 70.3%）和中性脂质（增加 24.32%）与对照相比有所提高。在添加 Fe、Zn 和 Mo 的条件下，活性氧（ROS）和抗氧化酶（SOD 和 POD）的产生增加，支持了脂质的积累。脂肪酸甲酯分析（FAME）表明，与对照相比，添加 Fe、Zn 和 Mo 后，培养基中 SFA 和 MUFA 的总体百分比增加，这对于优质生物柴油至关重要。此外，从 FAMEs 中得到的生物柴油特性，如 CN、SV、IV、CFPP、OS、υ、ρ 和 HHV，均符合生物柴油标准。
