Department of Dermatology, Leiden University Medical Centre, Leiden, The Netherlands.
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Eur J Dermatol. 2017 Jun 1;27(3):237-246. doi: 10.1684/ejd.2017.2984.
Papillary and reticular fibroblasts have different effects on keratinocyte proliferation and differentiation. The aim of this study was to investigate whether these effects are caused by differential secretion of soluble factors or by differential generation of extracellular matrix from papillary and reticular fibroblasts. To study the effect of soluble factors, keratinocyte monolayer cultures were grown in papillary or reticular fibroblast-conditioned medium. To study the effect of extracellular matrix, keratinocytes were grown on papillary or reticular-derived matrix. Conditioned medium from papillary or reticular fibroblasts did not differentially affect keratinocyte viability or epidermal development. However, keratinocyte viability was increased when grown on matrix derived from papillary, compared with reticular, fibroblasts. In addition, the longevity of the epidermis was increased when cultured on papillary fibroblast-derived matrix skin equivalents compared with reticular-derived matrix skin equivalents. The findings indicate that the matrix secreted by papillary and reticular fibroblasts is the main causal factor to account for the differences in keratinocyte growth and viability observed in our study. Differences in response to soluble factors between both populations were less significant. Matrix components specific to the papillary dermis may account for the preferential growth of keratinocytes on papillary dermis.
乳头层和网状层纤维母细胞对角质细胞的增殖和分化有不同的影响。本研究旨在探讨这些影响是否由可溶性因子的差异分泌或由乳头层和网状层纤维母细胞产生的细胞外基质的差异引起。为了研究可溶性因子的影响，将角质细胞单层培养物在乳头层或网状层纤维母细胞条件培养基中生长。为了研究细胞外基质的影响，将角质细胞在乳头层或网状层衍生的基质上生长。来自乳头层或网状层纤维母细胞的条件培养基对角质细胞活力或表皮发育没有差异影响。然而，与网状层纤维母细胞衍生的基质相比，当在源自乳头层的基质上生长时，角质细胞的活力增加。此外，与在网状层纤维母细胞衍生的基质表皮等效物上培养相比，在源自乳头层纤维母细胞衍生的基质表皮等效物上培养时，表皮的寿命延长。研究结果表明，乳头层和网状层纤维母细胞分泌的基质是造成我们研究中观察到的角质细胞生长和活力差异的主要因果因素。两种细胞群对可溶性因子的反应差异较小。乳头真皮特有的基质成分可能是角质细胞优先在乳头真皮上生长的原因。
