Bouchez Valérie, AlBitar-Nehmé Sami, Novikov Alexey, Guiso Nicole, Caroff Martine
Institut Pasteur, Unité de Prévention et Thérapies Moléculaires des Maladies Humaines, 25 rue du Dr Roux, 75724 Paris, France.
Institute for integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique (CEA), Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud, Université Paris-Saclay, 91405 Orsay, France.
Int J Mol Sci. 2017 May 18;18(5):1080. doi: 10.3390/ijms18051080.
can cause invasive infections but can also be isolated from the respiratory tract of patients with whooping-cough like symptoms. For the first time, we describe the lipid A structure of reference strain ATCC 51541 (alias NCTC12912 or CIP104394) and those of three French clinical isolates originating from blood (Bho1) or from respiratory samples (FR4020 and FR4101). They were investigated using chemical analyses, gas chromatography-mass spectrometry (GC-MS), and matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). The analyses revealed a common bisphosphorylated -(1→6)-linked d-glucosamine disaccharide with hydroxytetradecanoic acid in amide linkages. Similar to , and lipids A, the hydroxytetradecanoic acid at the C-2' position are carrying in secondary linkage a 2-hydroxytetradecanoic acid residue resulting of post-traductional biosynthesis modifications. The three clinical isolates displayed characteristic structural traits compared to the ATCC 51541 reference strain: the lipid A phosphate groups are more or less modified with glucosamine in the isolates and reference strain, but the presence of 10:0(3-OH) is only observed in the isolates. This trait was only described in and strains, as well as in isolates by the past. The genetic bases for most of the key structural elements of lipid A were analyzed and supported the structural data.
可引起侵袭性感染，但也可从有百日咳样症状患者的呼吸道中分离得到。我们首次描述了参考菌株ATCC 51541（别名NCTC12912或CIP104394）以及三株来自法国的临床分离株（一株源于血液（Bho1），两株源于呼吸道样本（FR4020和FR4101））的脂多糖A结构。使用化学分析、气相色谱 - 质谱联用（GC - MS）和基质辅助激光解吸电离质谱（MALDI - MS）对它们进行了研究。分析揭示了一种常见的双磷酸化 -（1→6）-连接的d - 葡糖胺二糖，其羟基十四烷酸以酰胺键连接。与[此处原文缺失相关菌株信息]、[此处原文缺失相关菌株信息]和[此处原文缺失相关菌株信息]的脂多糖A相似，C - 2' 位的羟基十四烷酸在二级连接中携带一个翻译后生物合成修饰产生的2 - 羟基十四烷酸残基。与ATCC 51541参考菌株相比，这三株临床分离株表现出特征性的结构特征：分离株和参考菌株中的脂多糖A磷酸基团或多或少都被葡糖胺修饰，但仅在分离株中观察到10:0(3 - OH)的存在。这一特征过去仅在[此处原文缺失相关菌株信息]和[此处原文缺失相关菌株信息]菌株以及[此处原文缺失相关菌株信息]分离株中被描述过。对脂多糖A的大多数关键结构元件的遗传基础进行了分析，结果支持了结构数据。
