Endotoxines, Structures et Activités, UMR 8621, GDR 3048, du CNRS, Institut de Génétique et Microbiologie, Université de Paris-Sud, 91405, Orsay, France.
Rapid Commun Mass Spectrom. 2011 Apr 30;25(8):1075-81. doi: 10.1002/rcm.4960. Epub 2011 Mar 29.
Bordetella bronchiseptica is a respiratory pathogen in mammal species and its cell surface lipopolysaccharide-endotoxin is a potent virulence factor. In order to better characterize the endotoxin structure to virulence relationships, we studied the lipid A structures of B. bronchiseptica isolates from human and rabbit origins as a function of their virulence phases. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has been widely used for the structural characterization of bacterial endotoxins and their lipid A moieties. This method combined with chemical analytical methods proved to be essential for the characterization of small samples and discrete but essential structural modifications. The occurrence of palmitate (C(16)) in the B. bronchiseptica lipid A structures is shown for the first time at two sites. Their presence was also demonstrated for the first time in correlation with the virulence phase of B. bronchiseptica clinical isolates. The recently identified glucosamine modifications of Bordetella lipids A are also reported in these isolates.
支气管败血波氏杆菌是一种哺乳动物呼吸道病原体，其细胞表面脂多糖内毒素是一种强大的毒力因子。为了更好地描述内毒素结构与毒力的关系，我们研究了来自人类和兔源的支气管败血波氏杆菌分离株的脂磷壁酸结构，作为其毒力阶段的一个功能。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）已广泛用于细菌内毒素及其脂磷壁酸部分的结构特征描述。这种方法与化学分析方法相结合，对于小样本和离散但必要的结构修饰的特征描述是必不可少的。首次在两个位置显示出支气管败血波氏杆菌脂磷壁酸结构中存在棕榈酸（C(16)）。它们的存在也首次与支气管败血波氏杆菌临床分离株的毒力阶段相关联。在这些分离株中还报告了最近确定的博德特氏菌脂类 A 的氨基葡萄糖修饰。
