Sobiech P, Adamiak Z, Holak P, Jastrzębski P, Rogowski J, Brzeziński M, Bury K, Jałyński M, Baumgartner W
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Pol J Vet Sci. 2017 Mar 28;20(1):45-49. doi: 10.1515/pjvs-2017-0007.
The aim of this study was to evaluate the suitability of thromboelastometry for the analysis of blood test results in goats after the use of hemostatic dressings to control massive bleeding. The study was carried out on 12 goats, 6 animals in each of two subgroups. In all experimental animals incision of the femoral artery was performed, and bleeding was controlled with QuikClot gauze in the first group and Celox gauze in the second group. Dressings were applied for 60 minutes. Blood samples for thromboelastometry were collected from the jugular vein before the incision and 60 min after the application of a dressing. Clotting time (CT), clot formation time (CFT), maximum clot firmness (MCF) and α angle (°) were measured in three standard ROTEM assays (system with generation of reaction curve, numerical parameters and size of the blood clot): intrinsic coagulation pathway (INTEM), extrinsic coagulation pathway (EXTEM) and functional fibrinogen (FIBTEM). Complete hemostasis of the injured femoral artery was found in all goats. No significant differences between pre- and post-incision thromboelastometric parameters were found in any tests in any of the groups, which indicates that the use of dressings was not associated with blood coagulation disorders. This study is the first to describe the use of thromboelastometry in goats for the assessment of clot formation and hemostatic disorders.
本研究的目的是评估血栓弹力图在使用止血敷料控制山羊大量出血后分析血液检测结果方面的适用性。该研究对12只山羊进行,分为两个亚组,每组6只动物。在所有实验动物中,均进行股动脉切开术,第一组用QuikClot纱布控制出血,第二组用Celox纱布控制出血。敷料应用60分钟。在切开前和应用敷料60分钟后,从颈静脉采集用于血栓弹力图检测的血样。在三种标准的血栓弹力图检测(生成反应曲线、数值参数和血凝块大小的系统)中测量凝血时间(CT)、凝块形成时间(CFT)、最大凝块硬度(MCF)和α角(°):内源性凝血途径(INTEM)、外源性凝血途径(EXTEM)和功能性纤维蛋白原(FIBTEM)。所有山羊的受伤股动脉均实现完全止血。在任何一组的任何检测中,切开前后的血栓弹力图参数均未发现显著差异,这表明敷料的使用与凝血障碍无关。本研究首次描述了在山羊中使用血栓弹力图评估凝块形成和止血障碍。