Dzimira S, Nizanski W, Madej J A
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Pol J Vet Sci. 2017 Mar 28;20(1):123-131. doi: 10.1515/pjvs-2017-0016.
Disorders of sex development (DSD) are rare in cats. They can be caused by chromosomal aberrations, gene mutations or other undefined factors. The aim of the present study was to compare the histological structure and immunohistochemical reactivity of testes in cats with DSD and in healthy cats. The research material consisted of the gonads of four cats - phenotypic males with an incorrect structure of the reproductive system. The control group consisted of the testes of four healthy cats - routinely castrated phenotypical males. The material was fixed with formalin and embedded in paraffin; the sections were stained with hematoxylin and eosin. The immunohistochemical investigation were performed using monoclonal and polyclonal antibodies directed against desmin, vimentin, actin of smooth muscles, S100 protein and MCM3 protein. The results obtained allow concluding that the testes of cats with DSD differed in certain respects, mainly in the number of blood vessels, from the normal testes. Moreover, the results of immunohistochemical examination indicate that in the testes of cats with DSD the number of supporting cells is lower, the amount of interstitial cells is comparable and spermatogenesis is correct es compared to those determined in the control gonads. The number of blood vessels in cats with DSD is reduced by about 30%. It confirms the recommendations for castration of these animals in order to eliminate the potential inheritance of sex development disorders.
性发育障碍（DSD）在猫中较为罕见。它们可能由染色体畸变、基因突变或其他不明因素引起。本研究的目的是比较患有DSD的猫和健康猫睾丸的组织结构及免疫组化反应性。研究材料包括四只猫的性腺——生殖系统结构异常的表型雄性。对照组由四只健康猫的睾丸组成——常规阉割的表型雄性。材料用福尔马林固定并石蜡包埋；切片用苏木精和伊红染色。免疫组化研究使用针对结蛋白、波形蛋白、平滑肌肌动蛋白、S100蛋白和MCM3蛋白的单克隆和多克隆抗体进行。所得结果表明，患有DSD的猫的睾丸在某些方面与正常睾丸不同，主要体现在血管数量上。此外，免疫组化检查结果表明，与对照性腺相比，患有DSD的猫的睾丸中支持细胞数量较少，间质细胞数量相当，精子发生正常。患有DSD的猫的血管数量减少了约30%。这证实了对这些动物进行阉割以消除性发育障碍潜在遗传的建议。
