Hirose Noriyuki, Hua Zhensheng, Kato Yuichi, Zhang Qiangqiang, Li Ruoyu, Nishimura Kazuko, Masuda Michiaki
Department of Microbiology, Dokkyo Medical University School of Medicine, Tochigi 321-0293, Japan.
BD Japan, Co., Ltd., Tokyo 107-0052, Japan.
Med Mycol. 2018 Apr 1;56(3):279-287. doi: 10.1093/mmy/myx039.
In this study, six strains of Prototheca isolated in China from human patients diagnosed as protothecosis and cows with mastitis were characterized by polymerase chain reaction (PCR) and nucleotide sequencing of the ribosomal RNA gene (rDNA) and by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). The results indicated that three strains isolated from the human patients were P. zopfii genotype 1, revealing the first cases of human protothecosis associated with P. zopfii genotype 1. The remaining three strains were shown to be P. zopfii genotype 2. Interestingly, one strain isolated from the cerebrospinal fluid of the human patient appeared to have both of the genotype 1- and 2-specific alleles in the small subunit (SSU) rDNA although it was classified by MALDI-TOF MS as genotype 2. For genotyping of certain strains of P. zopfii, it may be necessary to comprehensively evaluate the diversity in the SSU rDNA sequences and the MALDI-TOF MS results.
在本研究中，对从中国诊断为原藻病的人类患者以及患乳腺炎的奶牛中分离出的6株原藻进行了聚合酶链反应（PCR）、核糖体RNA基因（rDNA）的核苷酸测序以及基质辅助激光解吸/电离飞行时间质谱分析（MALDI-TOF MS）。结果表明，从人类患者中分离出的3株为魏氏原藻基因型1，这是首次发现与魏氏原藻基因型1相关的人类原藻病病例。其余3株为魏氏原藻基因型2。有趣的是，从人类患者脑脊液中分离出的1株虽然通过MALDI-TOF MS分类为基因型2，但在小亚基（SSU）rDNA中似乎同时具有基因型1和2的特异性等位基因。对于某些魏氏原藻菌株的基因分型，可能有必要综合评估SSU rDNA序列的多样性和MALDI-TOF MS结果。
