Zeng Xuanhao, Kudinha Timothy, Kong Fanrong, Zhang Qiang-Qiang
Division of Mycology, Huashan Hospital, Fudan University, Shanghai, China.
Charles Sturt University, Leeds Parade, Orange, NSW, Australia.
Front Microbiol. 2019 Mar 7;10:443. doi: 10.3389/fmicb.2019.00443. eCollection 2019.
commonly exists in the environment, and causes invasive infections (protothecosis) in humans. The morbidity of protothecosis has increased rapidly in recent years, especially in systemic infections of patients with an impaired immune system. The infection in immunocompromised patients has a poor prognosis due to limited understanding of the pathogenesis of the disease, as most previous studies mainly focused on classification and recognition of pathogenic strains. In this study, we constructed the genome and transcriptome of two pathogenic strains and one environmental strain, by next generation sequencing methods. Based on our preliminary gene expression findings, genes in pathogenic strains are significantly up-regulated in metabolism in peroxisome, such as glyoxylate cycle, which may improve the organism's resistance to the harsh environment in phagolysosome of macrophage and its ability to survive in an anaerobic environment. We also found some significant up-regulated genes, which are related to adherence and penetration in dermatophytes, and we speculate that this may enhance the virulence capacity of pathogenic strains. Finally, the genomes and transcriptomes of described here provide some base for further studies on the pathogenesis of this organism.
通常存在于环境中，并可导致人类发生侵袭性感染（原藻病）。近年来，原藻病的发病率迅速上升，尤其是在免疫系统受损患者的全身感染中。由于对该疾病发病机制的了解有限，免疫功能低下患者的感染预后较差，因为此前大多数研究主要集中在致病菌株的分类和识别上。在本研究中，我们通过下一代测序方法构建了两株致病菌株和一株环境菌株的基因组和转录组。基于我们初步的基因表达结果，致病菌株中的基因在过氧化物酶体代谢中显著上调，如乙醛酸循环，这可能提高生物体对巨噬细胞吞噬溶酶体中恶劣环境的抵抗力及其在厌氧环境中的生存能力。我们还发现了一些与皮肤癣菌的黏附和穿透相关的显著上调基因，我们推测这可能增强致病菌株的毒力。最后，这里描述的基因组和转录组为进一步研究该生物体的发病机制提供了一些基础。
