Toxic effects of NH-N on embryonic development of Bufo gargarizans and Rana chensinensis.

Although nitrogen fertilizer is commonly used worldwide, little information is currently available about NH-N toxicity on amphibians. This study determined the acute and chronic toxic effects of NH-N on two native Chinese amphibian species (Bufo gargarizans and Rana chensinensis), and compared the negative sensitivity of different embryos to NH-N. Static renewal aqueous exposures were performed using B. gargarizans and R. chensinensis embryos at Gosner stage 2 over 96 h. In terms of 96 h-LC, B. gargarizans and R. chensinensis embryos had significantly different responses to NH-N, and the latter was more sensitive to NH-N than the former. In the chronic toxicity test, exposure to 10 mg LNH-N or higher significantly decreased the hatching rate of embryos in both species. Significant increases in the abnormality rate of embryos at 50 mg LNH-N or higher were observed and morphological abnormalities were characterized by axial flexures, yolk sac edema, and hyperplasia in both species. Additionally, the total length of embryos decreased in a dose-dependent manner after exposure to NH-N. The results indicate that NH-N exposure can increase abnormality and inhibit the hatching and development of embryos in B. gargarizans and R. chensinensis.