Department of Zoology, Faculty of Science, South Valley University, Qena 83523, Egypt.
Department of Forensic Medicine and Veterinary Toxicology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
Biomed Pharmacother. 2017 Jul;91:1017-1021. doi: 10.1016/j.biopha.2017.04.124. Epub 2017 May 15.
Scorpion envenomation is a common medical problem in many countries; it is an important cause of morbidity and mortality. The venom of Leiurus quinquestriatus (LQ) is responsible for a number of deaths in children and adults. It has been stated that specific pathophysiological conditions such as generation of oxygen free radicals may trigger the onset of multiple organ dysfunction; therefore, the present study aimed to assess the oxidative stress mediated by LQ crude venom and its effect on the biochemical parameters in rats. Adult male Albino rats (250±30g body weight) were divided into three groups (n=5). In control group, rats were intraperitoneally (ip) injected with 50μL saline solution. Groups 2 and 3 were ip injected with 0.1mg/kg and 0.2mg/kg body weight of crude venom, respectively. Blood samples and liver tissues were harvested 1, 2 and 4h post-injection. Serum levels of glucose, cholesterol, creatinine, urea, uric acid and malondialdehyde increased significantly in envenomed animals within 1, 2 and 4h post-injection, compared to controls. However, the levels of total serum protein, albumin, globulin and triglycerides as well as catalase, glutathione peroxidase and super oxide dismutase in envenomed rats were significantly decreased compared to controls. We can conclude that LQ crude venom induces oxidative stress via reduction of antioxidant systems and alters some biochemical parameters of envenomed rats.
蝎子螫伤是许多国家常见的医学问题，是发病率和死亡率的重要原因。五毒蝎（LQ）的毒液是导致儿童和成人死亡的原因之一。有人指出，产生氧自由基等特定病理生理条件可能引发多器官功能障碍；因此，本研究旨在评估 LQ 粗毒液介导的氧化应激及其对大鼠生化参数的影响。成年雄性白化大鼠（体重 250±30g）分为三组（n=5）。在对照组中，大鼠腹膜内（ip）注射 50μL 生理盐水。第 2 组和第 3 组分别注射 0.1mg/kg 和 0.2mg/kg 体重的粗毒液。注射后 1、2 和 4 小时采集血样和肝组织。与对照组相比，注射后 1、2 和 4 小时，中毒动物血清中的葡萄糖、胆固醇、肌酐、尿素、尿酸和丙二醛水平显著升高。然而，与对照组相比，中毒大鼠的总血清蛋白、白蛋白、球蛋白和甘油三酯以及过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶的水平显著降低。我们可以得出结论，LQ 粗毒液通过降低抗氧化系统诱导氧化应激，并改变中毒大鼠的一些生化参数。
