Omran M A, Abdel-Rahman M S
Pharmacology and Toxicology Department, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark 07103-2714.
Toxicol Lett. 1992 Jun;61(1):99-109. doi: 10.1016/0378-4274(92)90068-u.
Egyptian scorpion venom was collected by electrical stimulation of the telson. Rats were injected with the lyophilized venom in 3 different doses (100, 200 and 400 micrograms/kg). Blood samples were drawn by heart puncture before and 4 h after venom administration. Serum was separated and collected for determination of glucose, blood urea nitrogen (BUN), creatinine, uric acid (UA), total proteins, cholesterol, sodium, potassium, calcium, inorganic phosphorus, alkaline phosphatase, aspartate aminotransferase (AST, GOT), alanine aminotransferase (ALT, GPT), lactate dehydrogenase and creatine phosphokinase (CPK). Serum glucose, creatinine, GOT, GPT and LDH were increased significantly in all treatments. At the same time serum BUN and CPK were elevated significantly with a dose-response relationship. On the other hand, serum total proteins, uric acid, cholesterol, calcium and potassium were significantly decreased 4 h after administration of the 3 doses. These changes in clinical chemistry parameters are most probably related to the toxic effect of the venom on the target organs.
通过对蝎尾进行电刺激来收集埃及蝎子毒液。给大鼠注射3种不同剂量（100、200和400微克/千克）的冻干毒液。在毒液给药前和给药后4小时通过心脏穿刺采集血样。分离并收集血清，用于测定葡萄糖、血尿素氮（BUN）、肌酐、尿酸（UA）、总蛋白、胆固醇、钠、钾、钙、无机磷、碱性磷酸酶、天冬氨酸转氨酶（AST，GOT）、丙氨酸转氨酶（ALT，GPT）、乳酸脱氢酶和肌酸磷酸激酶（CPK）。在所有处理组中，血清葡萄糖、肌酐、GOT、GPT和LDH均显著升高。同时，血清BUN和CPK显著升高，呈剂量反应关系。另一方面，在给予3种剂量毒液4小时后，血清总蛋白、尿酸、胆固醇、钙和钾显著降低。临床化学参数的这些变化很可能与毒液对靶器官的毒性作用有关。
