Department of Physiology and Pharmacology, China Pharmaceutical University, Nanjing, China.
National Experimental Teaching Demonstration Center of Pharmacy, China Pharmaceutical University, Nanjing, China.
Inflammation. 2017 Oct;40(5):1520-1531. doi: 10.1007/s10753-017-0593-6.
The purpose of the present study was to investigate the effects of salidroside (Sal) on lung injury in lipopolysaccharide (LPS)-induced endotoxemic in vitro and in vivo. SD rats were randomly divided into five groups: control group, LPS group (15 mg kg), LPS plus dexamethasone (2 mg kg), and LPS plus Sal groups with different Sal doses (20 mg kg, 40 mg kg). Wet-to-dry weight (W/D) ratio was performed. Hematoxylin-eosin (HE) staining of lung was performed. Lung level of myeloperoxidase (MPO) was measured. Serum levels of the activities of the anti-oxidant superoxide dismutase (SOD), glutathione peroxidase (GSH-px), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) were measured. Caveolin-1 and TLR/NF-κB pathway proteins were detected by Western blot. In vitro, we evaluated the protective effect of Sal on A549 cell line induced by LPS. The activities of the antioxidant SOD, CAT, GSH and GPX, TNF-α, IL-6, and IL-1β in cellular supernatant were measured. Caveolin-1 and TLR/NF-κB pathway was examined by Western blot. As a result, Sal significantly attenuated the above indices. In addition, Sal exerts pronounced protective effects in rats subjected to LPS possibly through inhibiting the caveolin-1 and TLR/NF-κB pathway in vivo. Our results indicated that Sal could be a potential therapeutic agent for the treatment of lung injury disease.
本研究旨在探讨红景天苷（Sal）对脂多糖（LPS）诱导的体外和体内内毒素血症肺损伤的影响。SD 大鼠随机分为五组：对照组、LPS 组（15mg/kg）、LPS 加地塞米松组（2mg/kg）和 LPS 加不同剂量 Sal 组（20mg/kg、40mg/kg）。进行湿重/干重（W/D）比值测定。进行肺组织髓过氧化物酶（MPO）水平测定。检测血清中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-px）、谷胱甘肽（GSH）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的活性。采用 Western blot 检测 Cav-1 和 TLR/NF-κB 通路蛋白。在体外，我们评估了 Sal 对 LPS 诱导的 A549 细胞系的保护作用。测定细胞上清液中抗氧化 SOD、CAT、GSH 和 GPX 的活性、TNF-α、IL-6 和 IL-1β。通过 Western blot 检测 Cav-1 和 TLR/NF-κB 通路。结果表明，Sal 显著减轻了上述指标。此外，Sal 在 LPS 处理大鼠中表现出明显的保护作用，可能是通过体内抑制 Cav-1 和 TLR/NF-κB 通路。我们的研究结果表明，Sal 可能是治疗肺损伤疾病的潜在治疗剂。
