Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan; Hitachi Zosen Corporation, 1-7-89 Nankokita, Suminoe-ku, Osaka 559-8559, Japan.
Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, No. 9 Section 4, Renmin Nan Road, Chengdu 610041, China; College of Architecture and Environment, Sichuan University, No. 24 South Section 1, First Ring Road, Chengdu 610065, China.
Waste Manag. 2017 Sep;67:86-94. doi: 10.1016/j.wasman.2017.04.030. Epub 2017 May 17.
Efficient ethanol production from waste paper requires the addition of expensive nutrients. To reduce the production cost of ethanol from waste paper, a study on how to produce ethanol efficiently by adding kitchen waste (potentially as a carbon source, nutrient source, and acidity regulator) to waste paper was performed and a process of successive liquefaction, presaccharification, and simultaneous saccharification and fermentation (L+PSSF) was developed. The individual saccharification performances of waste paper and kitchen waste were not influenced by their mixture. Liquefaction of kitchen waste at 90°C prior to presaccharification and simultaneous saccharification and fermentation (PSSF) was essential for efficient ethanol fermentation. Ethanol at concentrations of 46.6 or 43.6g/l was obtained at the laboratory scale after fermentation for 96h, even without pH adjustment and/or the addition of extra nutrients. Similarly, ethanol at a concentration of 45.5g/l was obtained at the pilot scale after fermentation for 48h. The ethanol concentration of L+PSSF of the mixture of waste paper and kitchen waste was comparable to that of PSSF of waste paper with added nutrients (yeast extract and peptone) and pH adjustment using HSO, indicating that kitchen waste is not only a carbon source but also an excellent nutrient source and acidity regulator for fermentation of the mixture of waste paper and kitchen waste.
从废纸中高效生产乙醇需要添加昂贵的营养物质。为了降低从废纸生产乙醇的生产成本，研究了通过添加厨房垃圾（可能作为碳源、营养源和酸度调节剂）到废纸中来高效生产乙醇的方法，并开发了连续液化、预糖化和同时糖化发酵（L+PSSF）工艺。废纸和厨房垃圾的单独糖化性能不受其混合物的影响。在预糖化和同时糖化发酵（PSSF）之前，在 90°C 下液化厨房垃圾对于高效乙醇发酵至关重要。在实验室规模下，发酵 96 小时后，甚至无需 pH 调节和/或添加额外的营养物质，就可获得 46.6 或 43.6g/l 的乙醇浓度。同样，在中试规模下发酵 48 小时后，可获得 45.5g/l 的乙醇浓度。废纸和厨房垃圾混合物的 L+PSSF 乙醇浓度可与添加营养物质（酵母提取物和蛋白胨）和使用 HSO 进行 pH 调节的 PSSF 废纸的乙醇浓度相媲美，这表明厨房垃圾不仅是碳源，而且是发酵废纸和厨房垃圾混合物的优良营养源和酸度调节剂。
