蛋白质消化组学分析揭示了鹿茸茸泡在模拟胃肠道消化中的生物活性。
Protein digestomic analysis reveals the bioactivity of deer antler velvet in simulated gastrointestinal digestion.
机构信息
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; Dalian Ocean University, Dalian 116023, China.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
出版信息
Food Res Int. 2017 Jun;96:182-190. doi: 10.1016/j.foodres.2017.04.002. Epub 2017 Apr 5.
Proteins are the most prominent bioactive component in deer antler velvet. The aim of the present study was to track the fate of protein of antler velvet by protein digestomics. The peptide profile identified by LC-MS/MS and the in vitro bioactivity of antler velvet aqueous extract (AAE) were investigated in simulated gastrointestinal digestion. A total of 23, 387 and 417 peptides in AAE, gastric and pancreatic digests were identified using LC-MS/MS, respectively. Collagens, the predominant proteins, released 34 peptides in gastric digests and 146 peptides in pancreatic digests. The gastric and pancreatic digests presented dipeptidyl peptidase IV (DPP-IV) and prolyl endopeptidase (PEP) inhibition activities. Four peptides from digests were proved to be DPP-IV and PEP inhibitory peptides. The results showed that the peptides released from antler velvet protein contributed to the bioactivity of antler velvet during digestion.
蛋白质是鹿茸中最主要的生物活性成分。本研究旨在通过蛋白质组学追踪鹿茸中蛋白质的命运。通过 LC-MS/MS 鉴定肽谱，并研究了鹿茸水提物（AAE）在模拟胃肠消化中的体外生物活性。分别使用 LC-MS/MS 鉴定了 AAE、胃消化液和胰消化液中的 23、387 和 417 种肽。胶原是主要蛋白质，在胃消化液中释放 34 种肽，在胰消化液中释放 146 种肽。胃消化液和胰消化液均具有二肽基肽酶 IV（DPP-IV）和脯氨酰内肽酶（PEP）抑制活性。从消化液中鉴定出的 4 种肽是 DPP-IV 和 PEP 抑制肽。结果表明，在消化过程中，鹿茸蛋白释放的肽有助于鹿茸的生物活性。
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