Hu Xianqiao, Fang Changyun, Lu Lin, Hu Zhanqiang, Shao Yafang, Zhu Zhiwei
China National Rice Research Institute, Hangzhou 310006, China; Laboratory of Quality & Safety Risk Assessment for Rice (Hangzhou), Ministry of Agriculture, Hangzhou 310006, China.
China National Rice Research Institute, Hangzhou 310006, China; Laboratory of Quality & Safety Risk Assessment for Rice (Hangzhou), Ministry of Agriculture, Hangzhou 310006, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Jul 15;1058:19-23. doi: 10.1016/j.jchromb.2017.05.001. Epub 2017 May 5.
Soluble sugars in rice are the main components affecting sweetness taste of rice. In this paper, an accurate, precise and rapid method for simultaneous determination of multi soluble sugars in rice by using ion chromatography equipped with pulsed amperometric detector was presented. Pretreatment and parameters of ion chromatography and pulsed amperometric detector were optimized. Regression coefficients (R) of 0.9998, 1.0000, 0.9979, 0.9998 and 0.9998 were obtained for glucose, fructose, sucrose, raffinose and maltose, respectively. The recovery ranges of five sugars were 92.9-112.0% for milled rice matrix. Repeatability and reproducibility of the method were 0.8-9.7% and 1.9-7.6%, respectively. Method LODs of 3.1-34.6μgg were obtained for soluble sugars in milled rice matrix.
水稻中的可溶性糖是影响稻米甜味的主要成分。本文提出了一种使用配备脉冲安培检测器的离子色谱法同时准确、精确且快速地测定水稻中多种可溶性糖的方法。对离子色谱和脉冲安培检测器的预处理及参数进行了优化。葡萄糖、果糖、蔗糖、棉子糖和麦芽糖的回归系数（R）分别为0.9998、1.0000、0.9979、0.9998和0.9998。对于精米粉基质，五种糖的回收率范围为92.9 - 112.0%。该方法的重复性和再现性分别为0.8 - 9.7%和1.9 - 7.6%。精米粉基质中可溶性糖的方法检出限为3.1 - 34.6μg/g。
