Endocrinology and Metabolism Research Center, Department of Gynecology and Obstetrics, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
Department of Gynecology and Obstetrics, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Biol Trace Elem Res. 2018 Feb;181(2):199-206. doi: 10.1007/s12011-017-1045-8. Epub 2017 May 21.
Selenium is known to exert multiple beneficial effects including anti-inflammatory actions. The aim of the study was to evaluate the effects of selenium supplementation on gene expression levels of inflammatory cytokines and vascular endothelial growth factor (VEGF) in women with gestational diabetes (GDM). This randomized double-blind, placebo-controlled trial was carried out among 40 subjects diagnosed with GDM aged 18-40 years old. Subjects were randomly allocated into two groups to receive either 200 μg/day selenium supplements (n = 20) or placebo (n = 20) for 6 weeks. Gene expression of inflammatory cytokines and VEGF were assessed in lymphocytes of GDM women with RT-PCR method. Results of RT-PCR indicated that after the 6-week intervention, compared with the placebo, selenium supplementation downregulated gene expression of tumor necrosis factor alpha (TNF-α) (P = 0.02) and transforming growth factor beta (TGF-β) (P = 0.01), and upregulated gene expression of VEGF (P = 0.03) in lymphocytes of patients with GDM. There was no statistically significant change following supplementation with selenium on gene expression of interleukin (IL)-1β and IL-8 in lymphocytes of subjects with GDM. Selenium supplementation for 6 weeks in women with GDM significantly decreased gene expression of TNF-α and TGF-β, and significantly increased gene expression of VEGF, but did not affect gene expression of IL-1β and IL-8. Clinical trial registration number http://www.irct.ir : IRCT201612045623N95.
硒具有多种有益作用，包括抗炎作用。本研究旨在评估补充硒对妊娠期糖尿病（GDM）妇女炎症细胞因子和血管内皮生长因子（VEGF）基因表达水平的影响。这项随机、双盲、安慰剂对照试验在 40 名年龄在 18-40 岁的 GDM 患者中进行。将受试者随机分为两组，分别接受 200μg/天的硒补充剂（n=20）或安慰剂（n=20），干预时间为 6 周。采用 RT-PCR 法检测 GDM 女性淋巴细胞中炎症细胞因子和 VEGF 的基因表达。RT-PCR 结果表明，与安慰剂组相比，6 周干预后，硒补充组 GDM 患者淋巴细胞中肿瘤坏死因子-α（TNF-α）（P=0.02）和转化生长因子-β（TGF-β）（P=0.01）的基因表达下调，血管内皮生长因子（VEGF）的基因表达上调（P=0.03）。GDM 患者淋巴细胞中白细胞介素（IL）-1β和 IL-8 的基因表达在补充硒后无统计学意义上的变化。在 GDM 女性中补充硒 6 周可显著降低 TNF-α 和 TGF-β 的基因表达，显著增加 VEGF 的基因表达，但不影响 IL-1β 和 IL-8 的基因表达。临床试验注册号：http://www.irct.ir：IRCT201612045623N95。
