补充硒对体外受精候选的不孕多囊卵巢综合征妇女胰岛素和脂质相关基因表达的影响：一项随机、双盲、安慰剂对照试验。
The Effects of Selenium Supplementation on Gene Expression Related to Insulin and Lipid in Infertile Polycystic Ovary Syndrome Women Candidate for In Vitro Fertilization: a Randomized, Double-Blind, Placebo-Controlled Trial.
机构信息
Infertility and Reproductive Health Research Center (IRHRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Preventative Gynecology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Biol Trace Elem Res. 2018 Jun;183(2):218-225. doi: 10.1007/s12011-017-1148-2. Epub 2017 Sep 5.
UNLABELLED
This study was conducted to evaluate the effects of selenium supplementation on gene expression related to insulin and lipid in infertile women with polycystic ovary syndrome (PCOS) candidate for in vitro fertilization (IVF). This randomized double-blind, placebo-controlled trial was conducted among 40 infertile women with PCOS candidate for IVF. Subjects were randomly allocated into two groups to intake either 200-μg selenium (n = 20) or placebo (n = 20) per day for 8 weeks. Gene expression levels related to insulin and lipid were quantified in lymphocytes of women with PCOS candidate for IVF with RT-PCR method. Results of RT-PCR demonstrated that after the 8-week intervention, compared with the placebo, selenium supplementation upregulated gene expression of peroxisome proliferator-activated receptor gamma (PPAR-γ) (1.06 ± 0.15-fold increase vs. 0.94 ± 0.18-fold reduction, P = 0.02) and glucose transporter 1 (GLUT-1) (1.07 ± 0.20-fold increase vs. 0.87 ± 0.18-fold reduction, P = 0.003) in lymphocytes of women with PCOS candidate for IVF. In addition, compared with the placebo, selenium supplementation downregulated gene expression of low-density lipoprotein receptor (LDLR) (0.88 ± 0.17-fold reduction vs. 1.05 ± 0.22-fold increase, P = 0.01) in lymphocytes of women with PCOS candidate for IVF. We did not observe any significant effect of selenium supplementation on gene expression levels of lipoprotein(a) [LP(a)] in lymphocytes of women with PCOS candidate for IVF. Overall, selenium supplementation for 8 weeks in lymphocytes of women with infertile PCOS candidate for IVF significantly increased gene expression levels of PPAR-γ and GLUT-1 and significantly decreased gene expression levels of LDLR, but did not affect LP(a).
CLINICAL TRIAL REGISTRATION NUMBER
http://www.irct.ir : IRCT201704245623N113.
本研究旨在评估补充硒对接受体外受精（IVF）的多囊卵巢综合征（PCOS）不孕妇女胰岛素和脂质相关基因表达的影响。这是一项随机、双盲、安慰剂对照试验，纳入了 40 名接受 IVF 的 PCOS 不孕妇女。受试者被随机分为两组，每天分别摄入 200-μg 硒（n=20）或安慰剂（n=20），干预 8 周。采用 RT-PCR 法检测接受 IVF 的 PCOS 不孕妇女淋巴细胞中与胰岛素和脂质相关的基因表达水平。RT-PCR 结果显示，与安慰剂相比，8 周干预后，硒补充剂可上调接受 IVF 的 PCOS 不孕妇女淋巴细胞中过氧化物酶体增殖物激活受体γ（PPAR-γ）（1.06±0.15 倍增加与 0.94±0.18 倍减少，P=0.02）和葡萄糖转运蛋白 1（GLUT-1）（1.07±0.20 倍增加与 0.87±0.18 倍减少，P=0.003）的基因表达。此外，与安慰剂相比，硒补充剂可下调接受 IVF 的 PCOS 不孕妇女淋巴细胞中低密度脂蛋白受体（LDLR）（0.88±0.17 倍减少与 1.05±0.22 倍增加，P=0.01）的基因表达。我们没有观察到硒补充对接受 IVF 的 PCOS 不孕妇女淋巴细胞中脂蛋白（a）[LP(a)]基因表达水平的任何显著影响。总之，在接受 IVF 的 PCOS 不孕妇女的淋巴细胞中补充硒 8 周，可显著增加 PPAR-γ和 GLUT-1 的基因表达水平，显著降低 LDLR 的基因表达水平，但对 LP(a)无影响。
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