Alsufyani Hadeel A, Docherty James R
Department of Physiology, Royal College of Surgeons in Ireland, 123 St. Stephen's Green, Dublin 2, Ireland; Department of Physiology, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
Department of Physiology, Royal College of Surgeons in Ireland, 123 St. Stephen's Green, Dublin 2, Ireland.
Eur J Pharmacol. 2017 Aug 15;809:203-208. doi: 10.1016/j.ejphar.2017.05.028. Epub 2017 May 18.
We have investigated gender differences in the effects of cathinone and the interaction with caffeine on temperature and movement activity in Wistar rats. Telemetry probes were implanted in rats under isoflurane anaesthesia, and 7 days later, temperature and activity were recorded in conscious unrestrained animals. Caffeine (10mg/lkg) or vehicle, and 30min later, cathinone (5mg/kg) or vehicle, were injected subcutaneously. Cathinone produced significant and marked increases in activity, and the response to cathinone was significantly greater in female animals. The combination of caffeine and cathinone causes a short lived potentiation followed by a prolonged inhibition of the activity response to cathinone. Cathinone alone had minor effects on temperature. However, the combination of caffeine and cathinone produced a significant acute rise in temperature only in male rats in the 90min after cathinone injection. Hence, cathinone caused greater increases in activity in female than in male rats. Secondly, caffeine produced an initial potentiation followed by a prolonged inhibition of the activity response to cathinone. Thirdly, cathinone in combination with caffeine significantly raised temperature acutely in male but not female rats. These differences highlight the need to carry out gender studies of the actions of stimulants.
我们研究了去甲伪麻黄碱的作用以及与咖啡因的相互作用对Wistar大鼠体温和运动活动的性别差异。在异氟烷麻醉下将遥测探头植入大鼠体内，7天后，在清醒自由活动的动物中记录体温和活动情况。皮下注射咖啡因（10mg/kg）或赋形剂，30分钟后，再注射去甲伪麻黄碱（5mg/kg）或赋形剂。去甲伪麻黄碱显著且明显地增加了活动量，并且雌性动物对去甲伪麻黄碱的反应明显更大。咖啡因和去甲伪麻黄碱的组合会导致短暂的增强作用，随后对去甲伪麻黄碱的活动反应出现长时间的抑制。单独使用去甲伪麻黄碱对体温影响较小。然而，咖啡因和去甲伪麻黄碱的组合仅在去甲伪麻黄碱注射后90分钟内使雄性大鼠体温出现显著的急性升高。因此，去甲伪麻黄碱对雌性大鼠活动量的增加作用大于雄性大鼠。其次，咖啡因产生了初始增强作用，随后对去甲伪麻黄碱的活动反应出现长时间的抑制。第三，去甲伪麻黄碱与咖啡因的组合在雄性而非雌性大鼠中显著急性升高体温。这些差异凸显了开展兴奋剂作用性别研究的必要性。
