Stoccoro Andrea, Di Bucchianico Sebastiano, Coppedè Fabio, Ponti Jessica, Uboldi Chiara, Blosi Magda, Delpivo Camilla, Ortelli Simona, Costa Anna Luisa, Migliore Lucia
Department of Translational Research and New Technologies in Medicine and Surgery, Laboratory of Medical Genetics, University of Pisa, Via Roma 55, Pisa 56126, Italy,; Doctoral School in Genetics, Oncology and Clinical Medicine, Department of Medical Biotechnologies, University of Siena, Siena, Italy.
Department of Translational Research and New Technologies in Medicine and Surgery, Laboratory of Medical Genetics, University of Pisa, Via Roma 55, Pisa 56126, Italy.
Toxicol Lett. 2017 Jul 5;276:48-61. doi: 10.1016/j.toxlet.2017.05.016. Epub 2017 May 18.
Titanium dioxide nanoparticles (TiO NP) are broadly used in a wide range of applications. Several studies have reported that TiO NP possess cytotoxic and genotoxic properties that could induce adverse health effects in humans. The FP7 Sanowork project was aimed to minimize occupational hazard and exposure to engineered nanomaterials (ENM), including TiO NP, through the surface modification in order to avoid possible adverse toxic effects for humans. In this study we investigated cytotoxicity, genotoxicity and epigenetic properties of TiO NP uncoated and coated with silica or citrate, as well as of the benchmark material P25. We used a panel of in vitro assays in the human lung epithelial cell line A549, in order to better understand if the remediation strategy adopted was able to counteract possible toxic effects of uncoated TiO NP. Our results showed that the uncoated TiO NP were both cytotoxic and genotoxic, and the remediation strategy adopted did not reduce the adverse effects of uncoated TiO NP. In particular, the presence of citrate was able to increase their cytotoxicity and genotoxicity, exerting also epigenotoxic effects, as evaluated by the marked reduction of LINE-1 methylation levels.
二氧化钛纳米颗粒（TiO NP）广泛应用于众多领域。多项研究报告称，TiO NP具有细胞毒性和基因毒性，可能会对人类健康产生不良影响。第七框架计划（FP7）的Sanowork项目旨在通过表面改性将职业危害以及接触工程纳米材料（包括TiO NP）的情况降至最低，以避免对人类可能产生的不良毒性影响。在本研究中，我们调查了未包覆以及包覆有二氧化硅或柠檬酸盐的TiO NP以及基准材料P25的细胞毒性、基因毒性和表观遗传特性。我们在人肺上皮细胞系A549中使用了一组体外试验，以便更好地了解所采用的修复策略是否能够抵消未包覆TiO NP可能产生的毒性作用。我们的结果表明，未包覆的TiO NP具有细胞毒性和基因毒性，并且所采用的修复策略并未降低未包覆TiO NP的不良影响。特别是，柠檬酸盐的存在能够增加它们的细胞毒性和基因毒性，通过LINE-1甲基化水平的显著降低评估，还会产生表观遗传毒性作用。
