Shi Liang, Cui Yazhou, Zhou Xiaoyan, Luan Jing, Wang Linli, Han Jinxiang
School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences.
Key Laboratory for Rare Disease Research of Shandong Province, Key Laboratory for Biotech Drugs of the Ministry of Health, Shandong Medical Biotechnological Center, Shandong Academy of Medical Sciences.
Biosci Trends. 2017 Jul 24;11(3):355-359. doi: 10.5582/bst.2017.01082. Epub 2017 May 20.
Embryonic stem cells (ESCs) technology has garnered worldwide attention for its therapeutic applications in vivo. Researchers have previously shown that non-viral induced pluripotent stem cells (iPSCs) can be generated from urine. As a potential alternative, Urinary iPSCs (UiPSCs) are highly similar to embryonic stem cells (ESCs) in many aspects such as morphology, expression of pluripotency markers and the capacity to develop into three germ layers in vitro and in vivo. However, the degree of gene expression similarity between iPSCs and ESCs has not been completely elucidated. In the present study, we performed a comparative study on the gene expression profile between UiPSCs and ESCs using microarray technology, and identified 19 differentially expressed genes. Furthermore, four genes associated with reprogramming and differentiation including neuronatin (NNAT), piwilike RNA-mediated gene silencing 2 (PIWIL2), early growth response 1 (EGR1) and TATA-box binding protein associated factor 9b (TAF9B) were validated by quantitative real-time PCR (qRT-PCR) assays. Our results indicate that compared with ESCs, UiPSCs demonstrated a different pathway in reprogramming and differentiation preference from ESCs, and can be used as a potential tool in disease modeling, drug discovery and regenerative medicine.
胚胎干细胞（ESCs）技术因其在体内的治疗应用而受到全球关注。研究人员此前已表明，可以从尿液中生成非病毒诱导多能干细胞（iPSCs）。作为一种潜在的替代方法，尿液诱导多能干细胞（UiPSCs）在许多方面与胚胎干细胞（ESCs）高度相似，如形态、多能性标志物的表达以及在体外和体内发育成三个胚层的能力。然而，iPSCs与ESCs之间基因表达的相似程度尚未完全阐明。在本研究中，我们使用微阵列技术对UiPSCs和ESCs之间的基因表达谱进行了比较研究，并鉴定出19个差异表达基因。此外，通过定量实时PCR（qRT-PCR）分析验证了与重编程和分化相关的四个基因，包括神经调节蛋白（NNAT）、piwi样RNA介导的基因沉默2（PIWIL2）、早期生长反应1（EGR1）和TATA盒结合蛋白相关因子9b（TAF9B）。我们的结果表明，与ESCs相比，UiPSCs在重编程和分化偏好方面表现出与ESCs不同的途径，并且可以用作疾病建模、药物发现和再生医学的潜在工具。
