Zhang Shuquan, Zhao Guanjie, Zhao Yi, Gu Rui, Peng Chuangang, Pu Zhe, Wu Minfei
Department of Orthopedics, Tianjin Nankai Hospital, Tianjin 300100, P.R. China.
Department of Nephrology, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China.
Oncol Lett. 2017 May;13(5):3517-3521. doi: 10.3892/ol.2017.5871. Epub 2017 Mar 17.
The expression and correlation analysis of the regenerating gene family member 4 (RegIV) and vascular endothelial growth factors (VEGF-A and VEGF-C) in metastatic spinal tumors were studied. Fifteen patients with metastatic spinal tumors who underwent operation in our hospital from January 2011 to January 2013 were selected into this study. The expression level of tumor tissues in patients with spinal metastasis and RegIV, VEGF-A and VEGF-C of the corresponding paracancer normal tissue samples were evaluated by immunohistochemical staining method and the correlation between the expression of RegIV, VEGF-A and VEGF-C was analyzed. qRT-PCR results showed that the expression of RegIV was increased (P<0.05) in paracancer normal tissues and spinal metastatic tumor tissues. Compared with normal tissues, expression of RegIV, VEGF-A and VEGF-C was higher in metastatic spinal tumor tissues and the difference had statistical difference (P<0.05). Spearman's correlation analysis showed that the expression of RegIV was positively correlated with VEGF-A (r=0.683, P<0.05); the expression of RegIV positively correlated with VEGF-C (r=0.717, P<0.05). Cox regression analysis showed that RegIV, VEGF-A, VEGF-C expression and microvessel density counts are prognostic factors affecting spine metastasis (P<0.05), RegIV expression affected the survival of patients with relative risk. The high expression of RegIV in spinal metastatic tumors may promote the expression of VEGF-A and VEGF-C to increase the microvascular density, promote angiogenesis, and accelerate the occurrence and progression of spinal metastatic tumors.
研究了再生基因家族成员4（RegIV）与血管内皮生长因子（VEGF - A和VEGF - C）在脊柱转移瘤中的表达及相关性分析。选取2011年1月至2013年1月在我院接受手术的15例脊柱转移瘤患者纳入本研究。采用免疫组织化学染色法评估脊柱转移瘤患者肿瘤组织及相应癌旁正常组织样本中RegIV、VEGF - A和VEGF - C的表达水平，并分析RegIV、VEGF - A和VEGF - C表达之间的相关性。qRT - PCR结果显示，RegIV在癌旁正常组织和脊柱转移瘤组织中的表达均升高（P<0.05）。与正常组织相比，RegIV、VEGF - A和VEGF - C在脊柱转移瘤组织中的表达更高，差异具有统计学意义（P<0.05）。Spearman相关性分析显示，RegIV的表达与VEGF - A呈正相关（r = 0.683，P<0.05）；RegIV的表达与VEGF - C呈正相关（r = 0.717，P<0.05）。Cox回归分析显示，RegIV、VEGF - A、VEGF - C表达及微血管密度计数是影响脊柱转移的预后因素（P<0.05），RegIV表达影响患者生存的相对风险。RegIV在脊柱转移瘤中的高表达可能促进VEGF - A和VEGF - C的表达，增加微血管密度，促进血管生成，加速脊柱转移瘤的发生和进展。
