Miller T, Dahl G, Werner R
Department of Biochemistry, University of Miami, FL 33101.
Biosci Rep. 1988 Oct;8(5):455-64. doi: 10.1007/BF01121644.
A genomic clone for the rat liver gap junction protein (connexin-32) was isolated and characterized by restriction enzyme mapping and sequence analysis. While the complete coding sequence is contained within one uninterrupted block, the 5'-untranslated region of the transcript contains a 6.1 kb intron. Both S1 nuclease protection and primer extension assays indicate multiple transcription start sites. Sequences homologous to cAMP response elements are found near the transcription start sites and within the 3'-end of the intron.
通过限制性内切酶图谱分析和序列分析，分离并鉴定了大鼠肝脏间隙连接蛋白（连接蛋白-32）的基因组克隆。虽然完整的编码序列包含在一个不间断的片段中，但转录本的5'-非翻译区含有一个6.1 kb的内含子。S1核酸酶保护试验和引物延伸试验均表明存在多个转录起始位点。在转录起始位点附近和内含子的3'-末端发现了与cAMP反应元件同源的序列。
