Werner R, Levine E, Rabadan-Diehl C, Dahl G
Department of Physiology and Biophysics, University of Miami School of Medicine, FL 33101.
Proc Natl Acad Sci U S A. 1989 Jul;86(14):5380-4. doi: 10.1073/pnas.86.14.5380.
The oocyte cell-cell channel assay was used to demonstrate that connexin-43 is a cell-cell channel-forming protein as previously shown for connexin-32. Expression of connexin-32 in one and connexin-43 in the other oocyte of a pair results in the formation of junctional conductances at rates similar to those observed when only one or the other connexin is expressed in both oocytes of a pair. This suggests that hybrid cell-cell channels form in the oocyte system. Hybrid channels also form when a connexin-43 mRNA-injected oocyte is paired with a noninjected oocyte expressing endogenous connexin. The latter hybrids have properties apparently contributed by both types of hemichannels. Pure connexin-43 channels are not voltage gated, whereas pure oocyte channels are voltage dependent; hybrids of these channels rectify.
卵母细胞细胞间通道分析用于证明连接蛋白43是一种细胞间通道形成蛋白，正如之前对连接蛋白32的研究所示。在一对卵母细胞中，一个表达连接蛋白32，另一个表达连接蛋白43，结果形成的连接电导速率与在一对卵母细胞中仅表达其中一种连接蛋白时所观察到的速率相似。这表明在卵母细胞系统中形成了杂合细胞间通道。当注射连接蛋白43 mRNA的卵母细胞与表达内源性连接蛋白的未注射卵母细胞配对时，也会形成杂合通道。后者的杂合通道具有明显由两种半通道共同作用产生的特性。纯连接蛋白43通道不是电压门控的，而纯卵母细胞通道是电压依赖性的；这些通道的杂合体具有整流作用。
