Ca-NIR：一种基于二羟基蒽-半菁荧光团的比率型近红外钙探针。
Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore.
作者信息
Collot Mayeul, Ponsot Flavien, Klymchenko Andrey S
机构信息
Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Faculté de Pharmacie, 74, Route du Rhin, 67401 ILLKIRCH Cedex, France.
出版信息
Chem Commun (Camb). 2017 Jun 1;53(45):6117-6120. doi: 10.1039/c7cc02418e.
Fluorescent calcium probes are essential tools for studying the fluctuation of calcium ions in cells. Herein, we developed Ca-NIR, the first ratiometric calcium probe emitting in the near infrared region. This probe arose from the fusion of a BAPTA chelator and a dihydroxanthene-hemicyanine fluorophore. It is efficiently excited with common 630-640 nm lasers and displays two distinct emission bands depending on the calcium concentration (K = ∼8 μM). The physicochemical and spectroscopic properties of Ca-NIR allowed for ratiometric imaging of calcium distribution in live cells.
荧光钙探针是研究细胞内钙离子波动的重要工具。在此，我们开发了Ca-NIR，这是首个在近红外区域发射的比率型钙探针。该探针由BAPTA螯合剂和二羟基蒽-半菁荧光团融合而成。它能被常见的630-640 nm激光有效激发，并根据钙浓度（K = ∼8 μM）显示出两个不同的发射带。Ca-NIR的物理化学和光谱性质使得对活细胞中钙分布进行比率成像成为可能。
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