软体动物平滑肌纤维收缩、强直收缩和舒张所诱导的核苷酸含量及能荷变化。采用反相离子对高效液相色谱法的分析

Changes of nucleotide contents and of energy charge induced by contraction, catch and relaxation in smooth molluscan muscle fibres. An analysis using reversed-phase ion-pair high-performance liquid chromatography.

Gies A

Freie Universität Berlin, Institut für Tierphysiologie, FRG.

Comp Biochem Physiol B. 1988;91(3):483-7. doi: 10.1016/0305-0491(88)90009-0.

Abstract

The content of 14 different nucleotides, including cAMP, in isolated muscle fibres of the anterior byssus retractor muscle of Mytilus edulis was analysed. The nucleotide levels were determined after the muscle fibres performed phasic, tonic, or tetanic contractions and after serotonin-induced relaxation of tonic contraction. 2. Isolated resting muscle fibres revealed a lower energy charge than freshly-dissected ones. 3. During active force development adenosine energy charge decreased to stay at the same low level during catch, tetanus and serotonin-induced relaxation of catch respectively. 4. The energy charges of the guanosine, uridine and cytidine systems did not show changes parallel to the adenosine system. 5. The levels of cyclic AMP were only changed under the influence of serotonin.

摘要

对紫贻贝前足丝牵缩肌分离出的肌纤维中14种不同核苷酸（包括环磷酸腺苷）的含量进行了分析。在肌纤维进行相位收缩、强直性收缩或强直收缩后，以及在5-羟色胺诱导的强直性收缩松弛后，测定了核苷酸水平。2. 分离出的静息肌纤维显示出比刚解剖的肌纤维更低的能荷。3. 在主动力发展过程中，腺苷能荷下降，在强直收缩、破伤风和5-羟色胺诱导的强直收缩松弛过程中分别保持在相同的低水平。4. 鸟苷、尿苷和胞苷系统的能荷没有显示出与腺苷系统平行的变化。5. 环磷酸腺苷的水平仅在5-羟色胺的影响下发生变化。