Sharma Seema, Saxena Dharmesh C, Riar Charanjit S
Department of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology, Longowal, 148106, Sangrur, Punjab, India.
Department of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology, Longowal, 148106, Sangrur, Punjab, India.
Food Chem. 2017 Oct 15;233:20-28. doi: 10.1016/j.foodchem.2017.04.099. Epub 2017 Apr 18.
A central composite rotatable design was applied to study the effects of soaking time, germination time and temperature on the responses; total phenolics, total flavonoids and antioxidant activity for the biochemical enhancement of bioactive components of Kodo millet. The optimum conditions for producing germinated Kodo millet flour of highest TPC (83.01mgGAE/100g), TFC (87.53mgRUE/g) and AoxA (91.34%), were soaking time (13.81h), germination temperature (38.75°C) and germination time (35.82h). Protein increased significantly form, 6.7 to 7.9%, dietary fibers from 35.30 to 38.34g/100g, minerals from 232.82 to 251.73mg/100g, GABA contents from 9.36 to 47.43mg/100g, whereas phytates and tannins decreased from 1.344 to 0.997mol/kg and 1.603 to 0.234mg/100g respectively, in optimized germinated Kodo millet sample. Six new bioactive compounds [n-propyl-9,12,15-octadecatrienoate (0.86%), pregan,20-one-2hydroxy,5,6,epox-15-methyl (3.45%), hexa-decanoicacid (8.19%), 9,O-ctadecenoicacid (5.00%), butyl-6,9,12,15-octadecatetraenoate (4.03%), hexadecanoicacid-methylester (1.43%)], synthesized as a result of germination under optimum conditions in the Kodo millet depicted the germination potential of millets as a source of valuable bioactive compounds.
采用中心复合旋转设计研究浸泡时间、发芽时间和温度对 responses 的影响；对龙爪稷生物活性成分进行生化强化的总酚、总黄酮和抗氧化活性。生产具有最高总酚含量（83.01mgGAE/100g）、总黄酮含量（87.53mgRUE/g）和抗氧化活性（91.34%）的发芽龙爪稷粉的最佳条件为浸泡时间（13.81 小时）、发芽温度（38.75°C）和发芽时间（35.82 小时）。在优化的发芽龙爪稷样品中，蛋白质含量从 6.7%显著增加到 7.9%，膳食纤维从 35.30g/100g 增加到 38.34g/100g，矿物质从 232.82mg/100g 增加到 251.73mg/100g，γ-氨基丁酸含量从 9.36mg/100g 增加到 47.43mg/100g，而植酸盐和单宁分别从 1.344mol/kg 降至 0.997mol/kg 和从 1.603mg/100g 降至 0.234mg/100g。在最佳条件下，龙爪稷发芽过程中合成的六种新生物活性化合物[正丙基-9,12,15-十八碳三烯酸酯（0.86%）、孕甾-20-酮-2-羟基-5,6-环氧-15-甲基（3.45%）、十六烷酸（8.19%）、反式-9-十八碳烯酸（5.00%）、丁基-6,9,12,15-十八碳四烯酸酯（4.03%）、十六烷酸甲酯（1.43%）]表明龙爪稷作为有价值生物活性化合物来源的发芽潜力。
