Division of Food and Nutrition, Chonnam National University, Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
Applied Technology & Research Division, R&D Center, AmorePacific Corporation, Yongin-si, Gyeonggi-do 446-729, Republic of Korea.
Food Chem. 2017 Oct 15;233:321-330. doi: 10.1016/j.foodchem.2017.04.079. Epub 2017 Apr 24.
Recently, we selected three tea (Camellia sinensis) cultivars that are rich in taste, epigallocatechin-3-O-gallate (EGCG) and epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG3″Me) and then cultivated them through asexual propagation by cutting in the same region. In the present study, proton nuclear magnetic resonance (H NMR)-based metabolomics was applied to characterize the metabotype and to understand the metabolic mechanism of these tea cultivars including wild type tea. Of the tea leaf metabolite variations, reverse associations of amino acid metabolism with catechin compound metabolism were found in the rich-taste, and EGCG- and EGCG3″Me-rich tea cultivars. Indeed, the metabolism of individual catechin compounds in the EGCG3″Me-rich cultivar differed from those of other tea cultivars. The current study highlights the distinct metabolism of various tea cultivars newly selected for cultivation and the important role of metabolomics in understanding the metabolic mechanism. Thus, comprehensive metabotyping is a useful method to assess and then develop a new plant cultivar.
最近，我们选择了三个口感浓郁、富含表没食子儿茶素没食子酸酯（EGCG）和表没食子儿茶素没食子酸酯-3-O-（3-O-甲基）酯（EGCG3″Me）的茶树品种，并通过无性繁殖在同一地区进行扦插繁殖。本研究采用基于质子核磁共振（H NMR）的代谢组学方法来描述这些茶树品种（包括野生型茶）的代谢表型，以了解其代谢机制。在茶叶代谢物的变化中，在口感浓郁、EGCG 和 EGCG3″Me 丰富的茶品种中发现了氨基酸代谢与儿茶素化合物代谢的反向关联。事实上，EGCG3″Me 丰富品种中个别儿茶素化合物的代谢与其他茶树品种不同。本研究强调了新选育的各种茶树品种的独特代谢，并突出了代谢组学在了解代谢机制方面的重要作用。因此，全面的代谢组学分析是评估和开发新植物品种的有效方法。
