Qu Hao, Long Yaqin, Wang Xuesong, Wang Kaibo, Chen Long, Yang Yunqiu, Chen Linbo
Tea Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650000, China.
Yunnan Provincial Key Laboratory of Tea Science, Tea Research Institute, Yunnan Academy of Agricultural Sciences, Menghai 666201, China.
Microorganisms. 2023 Aug 30;11(9):2188. doi: 10.3390/microorganisms11092188.
is a tea leaf moth that is considered one of the most destructive pests of (tea plant). Several recent studies have shown that many insects acquire part of the microbiome from their host and soil, but the pattern and diversity of their microbiome have not been clearly demonstrated. The present study aimed to investigate the bacterial and fungal communities present in the rhizospheric soil and leaf of tea plant compared to the gut of tea moth at different developmental stages (larvae, pupae, adult female and male) using Illumina MiSeq technology. Alpha diversity (Shannon index) showed higher ( < 0.05) bacterial and fungal diversity in soil samples than in leaf and tea moth larvae, pupae, and adult gut samples. However, during different developmental stages of tea moth, bacterial and fungal diversity did not differ ( > 0.05) between larvae, pupae, female, and male guts. Beta diversity also revealed more distinct bacterial and fungal communities in soil and leaf samples compared with tea moth gut samples, which had a more similar microbiome. Furthermore, Proteobacteria, Firmicutes, and Tenericutes were detected as the dominant bacterial phyla, while Ascomycota, Basidiomycota, and Mortierellomycota were the most abundant fungal phyla among all groups, but their relative abundance was comparatively higher ( < 0.05) in soil and leaf samples compared to tea moth gut samples. Similarly, , , and were the top three bacterial genera, while , , and were the top three fungal genera, and their relative abundance varied significantly ( < 0.05) among all groups. The KEGG analysis also revealed significantly higher ( < 0.5) enrichment of the functional pathways of bacterial communities in soil and leaf samples than in tea moth gut samples. Our study concluded that the bacterial and fungal communities of soil and tea leaves were more diverse and were significantly different from the tea moth gut microbiome at different developmental stages. Our findings contribute to our understanding of the gut microbiota of the tea moth and its potential application in the development of pest management techniques.
是一种茶树叶蛾，被认为是茶树最具破坏性的害虫之一。最近的几项研究表明，许多昆虫从其宿主和土壤中获取部分微生物群落，但其微生物群落的模式和多样性尚未得到明确证明。本研究旨在利用Illumina MiSeq技术，调查茶树根际土壤和叶片中与不同发育阶段（幼虫、蛹、成年雌蛾和雄蛾）茶蛾肠道中的细菌和真菌群落。α多样性（香农指数）表明，土壤样本中的细菌和真菌多样性高于叶片以及茶蛾幼虫、蛹和成虫肠道样本（P<0.05）。然而，在茶蛾的不同发育阶段，幼虫、蛹、雌蛾和雄蛾肠道之间的细菌和真菌多样性没有差异（P>0.05）。β多样性还显示，与茶蛾肠道样本相比，土壤和叶片样本中的细菌和真菌群落更明显不同，茶蛾肠道样本的微生物群落更相似。此外，变形菌门、厚壁菌门和柔膜菌门被检测为主要细菌门，而子囊菌门、担子菌门和被孢霉门是所有组中最丰富的真菌门，但与茶蛾肠道样本相比，它们在土壤和叶片样本中的相对丰度相对较高（P<0.05）。同样， 、 和 是前三大细菌属，而 、 和 是前三大真菌属，它们在所有组中的相对丰度差异显著（P<0.05）。KEGG分析还显示，土壤和叶片样本中细菌群落功能途径的富集程度显著高于茶蛾肠道样本（P<0.05）。我们的研究得出结论，土壤和茶叶中的细菌和真菌群落更加多样，并且与不同发育阶段的茶蛾肠道微生物群有显著差异。我们的研究结果有助于我们了解茶蛾的肠道微生物群及其在害虫管理技术开发中的潜在应用。
