Balmforth A J, Lyall F, Morton J I, Ball S G
Department of Biochemistry, University of Glasgow, U.K.
Eur J Pharmacol. 1988 Oct 18;155(3):305-8. doi: 10.1016/0014-2999(88)90519-5.
Incubation of cultured mesenteric vascular smooth muscle cells with dopamine, in the presence of propranolol, caused an increase in cyclic AMP formation in a concentration-dependent manner (Ka apparent 6.8 +/- 0.5 microM). This effect of dopamine was inhibited by the DA1-receptor antagonist SCH 23390 (Ki = 1 nM). These results suggest that cultured mesenteric vascular smooth muscle cells express DA1-receptors linked to adenylate cyclase.
在普萘洛尔存在的情况下，用多巴胺孵育培养的肠系膜血管平滑肌细胞，会导致环磷酸腺苷（cAMP）的生成以浓度依赖的方式增加（表观解离常数Ka为6.8±0.5微摩尔）。多巴胺的这种作用被DA1受体拮抗剂SCH 23390抑制（抑制常数Ki = 1纳摩尔）。这些结果表明，培养的肠系膜血管平滑肌细胞表达与腺苷酸环化酶相偶联的DA1受体。
