在大鼠精囊的场刺激制备物中，三磷酸腺苷（ATP）并非与去甲肾上腺素共同作为神经递质的证据。

Evidence that ATP does not act as a cotransmitter with noradrenaline in field-stimulated preparations of rat seminal vesicles.

作者信息

Chin P F, Pennefather J N

机构信息

Department of Pharmacology, Monash University, Victoria, Australia.

出版信息

Eur J Pharmacol. 1988 Oct 18;155(3):309-12. doi: 10.1016/0014-2999(88)90520-1.

DOI:10.1016/0014-2999(88)90520-1

PMID:2853068

Abstract

Field stimulation (20 pulses, 60 V, 2 ms, 5-70 Hz) of the nerve terminals within the rat seminal vesicle produced monophasic contractions which were blocked by guanethidine 5 microM and phentolamine 1 microM, and minimal in tissues from rats pretreated with reserpine, but unaffected by repeated application of alpha,beta-methylene ATP. Field stimulation at 40 Hz led to the release of ATP (0.39 +/- 0.12 pmol/mg; n = 10). However neither ATP nor alpha,beta-methylene ATP 10 microM caused contraction of the tissue. We conclude that ATP does not act as a cotransmitter in preparations of rat seminal vesicle because P2-purinoceptors appear to be absent.

摘要

对大鼠精囊内神经末梢进行场刺激（20个脉冲，60伏，2毫秒，5 - 70赫兹）可产生单相收缩，这种收缩被5微摩尔的胍乙啶和1微摩尔的酚妥拉明阻断，在用利血平预处理的大鼠组织中收缩最小，但不受重复应用α,β-亚甲基ATP的影响。40赫兹的场刺激导致ATP释放（0.39±0.12皮摩尔/毫克；n = 10）。然而，10微摩尔的ATP和α,β-亚甲基ATP均未引起组织收缩。我们得出结论，在大鼠精囊制剂中ATP不作为共递质起作用，因为似乎不存在P2嘌呤受体。