Li B W, Andrews K W
U.S. Department of Agriculture, Beltsville Human Nutrition Research Center, MD 20705.
J Assoc Off Anal Chem. 1988 Sep-Oct;71(5):1063-4.
A simplified method, based on the same principles as the AOAC enzymatic-gravimetric method for determining total dietary fiber (TDF) (43.A14-43.A20), has been tested on 12 food samples which had been used in other collaborative studies. TDF values obtained in our laboratory for these 2 methods were in good agreement (y = 0.96x + 0.39; r = 0.999). The simplified method uses a single incubation period and only 1 enzyme (amyloglucosidase), and thus yields smaller blank and ash corrections but a higher protein correction.
