Nishimune T, Sumimoto T, Yakusiji T, Kunita N, Ichikawa T, Doguchi M, Nakahara S
Osaka Prefectural Institute of Public Health, Higashinari, Japan.
J Assoc Off Anal Chem. 1991 Mar-Apr;74(2):350-9.
Total dietary fiber was determined in Japanese foods by the Prosky-AOAC method. To accomplish the analyses of unsuitable samples, we introduced a few minor modifications to the versions for (i) seaweed and fruits, (ii) cereals, and (iii) fish and meats. These modified methods were used together with the standard method to obtain results with reasonably good relative standard deviation for 231 foods and 21 groups of mixed foods. In this study, dietary fiber was defined so as not to exclude the nondigestible polysaccharide portions of animal foods. A method was proposed which could estimate more accurately the fiber components of animal foods by measuring the "nondigestible protein" of the fiber sample of the fiber sample by the Biuret colorimetric method, instead of the Kjeldahl method, to avoid deducting the values for aminopolysaccharides. In Japanese diets, the amount of fiber obtained from animals foods was less than 5% of the total intake of dietary fiber.
采用普罗茨基-美国公职分析化学师协会(Prosky-AOAC)方法测定日本食品中的总膳食纤维。为了完成对不合适样品的分析,我们对以下几类样品的分析方法进行了一些小的改进:(i)海藻和水果,(ii)谷物,以及(iii)鱼类和肉类。这些改进后的方法与标准方法一起用于分析231种食品和21组混合食品,从而获得相对标准偏差相当小的结果。在本研究中,膳食纤维的定义不排除动物食品中不可消化的多糖部分。提出了一种方法,通过用双缩脲比色法而非凯氏定氮法测量纤维样品中的“不可消化蛋白质”,可以更准确地估算动物食品中的纤维成分,以避免扣除氨基多糖的值。在日本饮食中,从动物食品中获取的纤维量不到膳食纤维总摄入量的5%。