Kajihara J, Enomoto M, Katoh K, Mitsuta K, Kohno M
Research Laboratories, Nippon Kayaku Co., Ltd., Tokyo.
J Biochem. 1988 Nov;104(5):855-7. doi: 10.1093/oxfordjournals.jbchem.a122563.
The relation between ESR-detectable Cu(II) and Cu,Zn-superoxide dismutase activity was examined. The Cu(II) spin numbers per one unit of SOD were 6.26 X 10(12) (+/- 0.51 X 10(12] spins in several preparations of recombinant human Cu,Zn-SOD, native placental, and erythrocyte SOD. Measurement could be performed over a wide range of pH (4.0-10.0), preferably at temperatures below -40 degrees C. The data obtained by this method correlated well to the results obtained by the method of Fridovich et al. using the xanthine-xanthine oxidase system (correlation coefficient 0.995). The specific activity of SOD was proportional to the Cu(II) content measured by ESR, but not to the total Cu content measured by atomic absorption. This indicates that it is important to measure the Cu(II) content for determining Cu,Zn-SOD activity.
研究了红细胞沉降率（ESR）可检测的铜（II）与铜锌超氧化物歧化酶活性之间的关系。在几种重组人铜锌超氧化物歧化酶、天然胎盘超氧化物歧化酶和红细胞超氧化物歧化酶制剂中，每单位超氧化物歧化酶的铜（II）自旋数为6.26×10¹²（±0.51×10¹²）自旋。测量可在较宽的pH范围（4.0 - 10.0）内进行，最好在低于 - 40℃的温度下进行。通过该方法获得的数据与弗里多维奇等人使用黄嘌呤 - 黄嘌呤氧化酶系统获得的结果相关性良好（相关系数0.995）。超氧化物歧化酶的比活性与通过电子顺磁共振（ESR）测量的铜（II）含量成正比，但与通过原子吸收测量的总铜含量不成正比。这表明测量铜（II）含量对于确定铜锌超氧化物歧化酶活性很重要。
