Sugiura M, Adachi T, Inoue H, Ito Y, Hirano K
J Pharmacobiodyn. 1981 Apr;4(4):235-44. doi: 10.1248/bpb1978.4.235.
Copper and zinc-containing superoxide dismutase (Cu,Zn-SOD), and manganese-containing superoxide dismutase (Mn-SOD) were purified from human placenta by heat treatment at 70 degrees for 5 min, following precipitation with ammonium sulfate and column chromatographies. The molecular weights of Cu,Zn-SOD and Mn-SOD estimated by gel filtration were 32000 and 81000, respectively. From the result of SDS polyacrylamide gel electrophoresis, Cu,Zn-SOD consisted of two subunits with an equal molecular weight of 16000 and Mn-SOD consisted of four subunits with an equal molecular weight of 21000. One g-atom each of copper and zinc was contained in the subunit of Cu,Zn-SOD, and one g-atom of manganese was contained in the subunit of Mn-SOD. Immunological properties of superoxide dismutases showed that no organ specificity existed but that a species specificity obviously existed. The method of Laurell's rocket immunoelectrophoresis which can determine Cu,Zn-SOD or Mn-SOD on the basis of the immunological difference has been established. The contents of Cu,Zn-SOD and Mn-SOD in various human tissues were measured by this method.
通过硫酸铵沉淀和柱色谱法，继以70℃热处理5分钟，从人胎盘中纯化出含铜锌超氧化物歧化酶（Cu,Zn-SOD）和含锰超氧化物歧化酶（Mn-SOD）。通过凝胶过滤法估算，Cu,Zn-SOD和Mn-SOD的分子量分别为32000和81000。SDS聚丙烯酰胺凝胶电泳结果显示，Cu,Zn-SOD由两个分子量均为16000的亚基组成，Mn-SOD由四个分子量均为21000的亚基组成。Cu,Zn-SOD的亚基中各含1克原子的铜和锌，Mn-SOD的亚基中含1克原子的锰。超氧化物歧化酶的免疫学特性表明，不存在器官特异性，但明显存在物种特异性。已建立基于免疫学差异测定Cu,Zn-SOD或Mn-SOD的劳雷尔火箭免疫电泳法。用该方法测定了各种人体组织中Cu,Zn-SOD和Mn-SOD的含量。
