Zhang Hao, Tao Yanling, Liu Haihui, Ren Saisai, Zhang Bin, Chen Hu
Department of Hematology, Affiliated Hospital of Jining Medical University, No. 79, Guhuai Road, Jining, Shandong 272000, China; Hematopoietic Stem Cell Transplantation, Affiliated Hospital to Academy of Military Medical Sciences, No. 8, East Street, Fengtai District, Beijing 100071, China.
Department of Pediatrics, The Affiliated Hospital of Jining Medical College, Jining, Shandong 272000, China.
Mol Immunol. 2017 Jul;87:293-299. doi: 10.1016/j.molimm.2017.03.003. Epub 2017 May 19.
Bone marrow derived mesenchymal stem cells (MSCs) play a critical role in immune modulation. However, immunomodulatory function of whole human umbilical cord derived mesenchymal stem cells (UC-MSCs) remains unclear. In this study, UC-MSCs were separated from whole umbilical cord using a single enzyme digestion. UC-MSCs (CD73, CD90, CD105, and CD34, CD45, HLA-DR) were differentiated into adipocytes, osteocytes and chondrocytes in vitro under specific stimulatory environments. UC-MSCs suppressed umbilical cord blood lymphocyte proliferation stimulated by mitogen, and ELISA showed that the secretion of INF-γ was downregulated, and the secretion of IL-4 was upregulated, with CD8 T cells markedly decreased and CD4 T cells changed lightly. Moreover, the infusion of UC-MSCs in recipient mice transplanted with donor bone marrow cells ameliorated acute graft-versus host disease (aGVHD) and extended survival. In conclusion, UC-MSCs might negatively modulate immunoreactions, and have application potential in the treatment of aGVHD caused by allogeneic stem cells transplantation.
骨髓间充质干细胞（MSCs）在免疫调节中发挥着关键作用。然而，人脐带间充质干细胞（UC-MSCs）的免疫调节功能仍不清楚。在本研究中，采用单一酶消化法从人脐带中分离出UC-MSCs。UC-MSCs（CD73、CD90、CD105以及CD34、CD45、HLA-DR）在特定刺激环境下于体外分化为脂肪细胞、骨细胞和软骨细胞。UC-MSCs抑制了由丝裂原刺激的脐带血淋巴细胞增殖，酶联免疫吸附测定（ELISA）显示，γ-干扰素（INF-γ）的分泌下调，白细胞介素-4（IL-4）的分泌上调，CD8 T细胞显著减少，CD4 T细胞变化轻微。此外，将UC-MSCs注入移植了供体骨髓细胞的受体小鼠体内，可改善急性移植物抗宿主病（aGVHD）并延长生存期。总之，UC-MSCs可能对免疫反应具有负向调节作用，在治疗异基因干细胞移植引起的aGVHD方面具有应用潜力。
Acta Biochim Biophys Sin (Shanghai). 2014-12
Stem Cell Res Ther. 2022-9-5
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