Erol Ayşe, Sel Figen Abatay, Süleymanoğlu Mediha, Demirayak Gökhan, Kuruca Dürdane Serap, Savran Oğuz Fatma
Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Department of Medical Biology, Institute of Health Sciences, Istanbul University, Istanbul, Turkey.
Mol Biol Rep. 2023 May;50(5):4347-4356. doi: 10.1007/s11033-023-08374-9. Epub 2023 Mar 20.
Mesenchymal stem cells (MSCs), are a novel therapeutic option as the most common cell source, play an important role in the immunomodulation. In this study, it was aimed to determine the effect of MSCs on cytokines secreted by the immune system cells.
Intracellular cytokine levels (Interleukin-4 (IL-4), Interferon-γ (IFN-γ), and Interleukin-17 (IL-17)) detected by flow cytometry before and after co-culture between peripheral blood mononuclear cells (PBMCs) and MCSs. At the same time, supernatant cytokine levels were measured using the ELISA.
In our study, MSCs were isolated from cord blood (CB) and Wharton's Jelly (WJ), and their surface markers (CD44 (100%), CD73 (99.6%), CD90 (100%), CD105 (88%)) shown by flow cytometry method. Both CB-MSCs and WJ-MSCs were used in co-culture MSC/PBMC ratios of 1/5 and 1/10, incubation times of 24 h and 72 h. In the present study, when we compared co-cultures of CB-MSC or WJ-MSC with PBMCs, intracellular levels of cytokines IFN-γ, IL-17 (pro-inflamatory) and IL-4 (anti-inflamatory) were increased, and supernatant levels were decreased significantly (p < 0.05). The level of transforming growth factor beta (TGF-β) (anti-inflamatory) was significantly decreased for both CB-MSC and WJ-MSC in supernatant (p < 0.05).
It was investigated pro-inflammatory and anti-inflammatory effects of CB-MSCs and WJ-MSCs on PBMCs with the obtained results. According to the results, MSCs demonstrated different immunologic effects after the incubation time and ratios. For further studies, it should be known between interaction of MSCs and immune system.
间充质干细胞（MSCs）作为最常见的细胞来源，是一种新型治疗选择，在免疫调节中发挥重要作用。本研究旨在确定MSCs对免疫系统细胞分泌的细胞因子的影响。
通过流式细胞术检测外周血单个核细胞（PBMCs）与间充质干细胞（MCSs）共培养前后细胞内细胞因子水平（白细胞介素-4（IL-4）、干扰素-γ（IFN-γ）和白细胞介素-17（IL-17））。同时，使用酶联免疫吸附测定法测量上清液细胞因子水平。
在我们的研究中，从脐带血（CB）和华通氏胶（WJ）中分离出MSCs，并通过流式细胞术方法显示其表面标志物（CD44（100%）、CD73（99.6%）、CD90（100%）、CD105（88%））。CB-MSCs和WJ-MSCs均用于共培养，MSC/PBMC比例为1/5和1/10，孵育时间为24小时和72小时。在本研究中，当我们将CB-MSC或WJ-MSC与PBMCs的共培养进行比较时，细胞内细胞因子IFN-γ、IL-17（促炎）和IL-4（抗炎）水平升高，而上清液水平显著降低（p<0.05）。CB-MSC和WJ-MSC在上清液中的转化生长因子β（TGF-β）（抗炎）水平均显著降低（p<0.05）。
通过获得的结果研究了CB-MSCs和WJ-MSCs对PBMCs的促炎和抗炎作用。根据结果，MSCs在孵育时间和比例后表现出不同的免疫效应。对于进一步的研究，应该了解MSCs与免疫系统之间相互作用。
