Department of Chemistry, Faculty of Science, University of Kurdistan, P.O. Box 416, 66177-15175 Sanandaj, Iran.
Department of Chemistry, Faculty of Science, University of Kurdistan, P.O. Box 416, 66177-15175 Sanandaj, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Oct 5;185:27-34. doi: 10.1016/j.saa.2017.05.034. Epub 2017 May 17.
We have described a simple and reliable colorimetric method for the sensing of biothiols such as cysteine, homocysteine, and glutathione in biological samples. The selective binding of chitosan capped silver nanoparticles to biothiols induced aggregation of the chitosan-Ag NPs. But the other amino acids that do not have thiol group cannot aggregate the chitosan-Ag NPs. Aggregation of chitosan-Ag NPs has been confirmed with UV-vis absorption spectra, zeta potential and transmission electron microscopy images. Under optimum conditions, good linear relationships existed between the absorption ratios (at A/A) and the concentrations of cysteine, homocysteine, and glutathione in the range of 0.1-10.0μM with detection limits of 15.0, 84.6 and 40.0nM, respectively. This probe was successfully applied to detect these biothiols in biological samples (urine and plasma).
我们描述了一种简单可靠的比色法，用于检测生物样品中的生物硫醇，如半胱氨酸、同型半胱氨酸和谷胱甘肽。壳聚糖包覆的银纳米粒子与生物硫醇的选择性结合诱导壳聚糖-Ag NPs 的聚集。但是其他没有巯基的氨基酸不能聚集壳聚糖-Ag NPs。壳聚糖-Ag NPs 的聚集已经通过紫外-可见吸收光谱、zeta 电位和透射电子显微镜图像得到证实。在最佳条件下，吸收比（A/A）与半胱氨酸、同型半胱氨酸和谷胱甘肽的浓度在 0.1-10.0μM 范围内呈良好的线性关系，检测限分别为 15.0、84.6 和 40.0nM。该探针成功应用于检测生物样品（尿液和血浆）中的这些生物硫醇。
