Department of Applied Chemistry, Cochin University of Science and Technology, Kochi 682 022, India.
Department of Applied Chemistry, Cochin University of Science and Technology, Kochi 682 022, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 5;188:113-119. doi: 10.1016/j.saa.2017.06.040. Epub 2017 Jul 2.
A sensor for the detection and determination of bio-thiols (glutathione (GSH) and cysteamine (Cyste)) has been developed by integrating the distinguished distance related optical characteristics of silver nanoparticles with the simplicity of colorimetric technique. In presence of these analytes, shift in surface plasmon resonance (SPR) absorption of silver nanoparticles (AgNPs) with change in its colour was observed. Yellow coloured AgNPs solution becomes colourless in presence of GSH and changes to red in presence of Cyste. FTIR, TEM and DLS studies were used to confirm the mechanism. The difference in absorption of AgNPs in the absence and presence of GSH was found to vary linearly in the range 1.00×10M to 5.00×10M concentration range with limit of detection at 3.68×10M. The method can also be applied to quantify Cyste in the range 1.10×10M to 5.00×10M with limit of detection at 1.80×10M. The utility of the proposed colorimetric assay is validated by determination of GSH and Cyste in artificial blood serum.
一种用于检测和测定生物硫醇（谷胱甘肽（GSH）和半胱胺（Cyste））的传感器已经通过将银纳米粒子的独特的距离相关光学特性与比色技术的简单性相结合而开发出来。在存在这些分析物的情况下，观察到银纳米粒子（AgNPs）的表面等离子体共振（SPR）吸收的位移及其颜色的变化。在 GSH 的存在下，黄色的 AgNPs 溶液变为无色，而在 Cyste 的存在下变为红色。FTIR、TEM 和 DLS 研究用于证实该机制。在不存在和存在 GSH 的情况下，AgNPs 的吸收差异在 1.00×10M 至 5.00×10M 的浓度范围内呈线性变化，检测限为 3.68×10M。该方法还可用于定量测定 1.10×10M 至 5.00×10M 范围内的 Cyste，检测限为 1.80×10M。通过在人工血清中测定 GSH 和 Cyste，验证了所提出的比色测定法的实用性。
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