Paris Jean-Baptiste, Seyer Damien, Jouenne Thierry, Thébault Pascal
Normandie Univ, UNIROUEN, INSA Rouen, CNRS, PBS, 76000 Rouen, France.
UnivCergyPontoise, LabERRMECe, EA1391, F-95302 Cergy-Pontoise, France.
Colloids Surf B Biointerfaces. 2017 Aug 1;156:186-193. doi: 10.1016/j.colsurfb.2017.05.025. Epub 2017 May 13.
Antibacterial polyolefins surfaces, combining biocidal and antiadhesive properties, were elaborated by a covalent grafting of antimicrobial peptides (AMPs), able to kill adherent bacteria, on a pre-immobilized hyaluronic acid (HA) layer, able to repel the micro-organisms. Different HA activation rate for its immobilization, were used to change HA layer morphology and number of residual free carboxylic acid functions for AMPs grafting. Based on adhesion tests on Staphylococcus epidermidis and microscopy fluorescent observations, the presence of the two combined properties was shown to be depended on the HA activation rate. Thus, the best addition effect was observed for an AMP grafting on a surface based on a high HA activation, data pointing out a decrease of the bacterial adhesion up to 99.8% and a perturbation of the bacterial membrane integrity of adhered bacteria. On the contrary, a decrease of the antibacterial activity was observed for an AMP grafting on a surface based on a low HA activation.
通过将能够杀死黏附细菌的抗菌肽（AMPs）共价接枝到能够排斥微生物的预先固定的透明质酸（HA）层上，制备了具有杀菌和抗黏附特性的抗菌聚烯烃表面。使用不同的HA固定活化率来改变HA层的形态以及用于AMPs接枝的残余游离羧酸官能团的数量。基于对表皮葡萄球菌的黏附测试和显微镜荧光观察，结果表明这两种组合特性的存在取决于HA活化率。因此，在基于高HA活化的表面上进行AMPs接枝时观察到了最佳的添加效果，数据表明细菌黏附减少了99.8%，并且黏附细菌的细胞膜完整性受到了干扰。相反，在基于低HA活化的表面上进行AMPs接枝时，观察到抗菌活性降低。
