CNRS, UMR 6270, Polymères, Biopolymères, Surfaces Laboratory, F-76820 Mont-Saint-Aignan, France; Normandie Univ, UR, France.
Colloids Surf B Biointerfaces. 2013 Dec 1;112:229-36. doi: 10.1016/j.colsurfb.2013.07.061. Epub 2013 Aug 16.
Bacterial adhesion leading to biofilm formation on the surface of implants is responsible for pathogenesis infections. One promising strategy to reduce the risk of infection consists of modifying implant surfaces by antibacterial coating. In the present study, the ability of ulvan, a non biocidal algal polysaccharide, to limit bacterial adhesion on titanium was investigated. To this end, titanium surfaces were modified by two different ulvans. Polysaccharides were covalently immobilized on titanium surfaces which had been previously functionalized by self assembled monolayers of aminoundecyltrimethoxysilane (AUTMS). Each step in the modification process was characterized by contact angle, X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). Bacterial adhesion assays showed that immobilized ulvans on titanium surface strongly decreased by about 90% the adhesion of Pseudomonas aeruginosa. Moreover, AFM observations showed that the polysaccharide also inhibited the bacterial spreading on the surface but not cell-to-cell interaction. The permanence of the anti-adhesive effect of the surfaces was finally tested on a non-motile organism, i.e., Staphylococcus epidermidis. The results showed that the effect was maintained for at least 24h.
细菌黏附导致植入物表面生物膜的形成是发病感染的原因。一种降低感染风险的有前途的策略是通过抗菌涂层来修饰植入物表面。在本研究中，研究了非杀菌海藻多糖岩藻聚糖在限制钛上细菌黏附方面的能力。为此，通过两种不同的岩藻聚糖对钛表面进行了修饰。多糖通过自组装的氨丙基三甲氧基硅烷（AUTMS）单层预先功能化的钛表面进行共价固定。修饰过程中的每一步都通过接触角、X 射线光电子能谱（XPS）和原子力显微镜（AFM）进行了表征。细菌黏附实验表明，固定在钛表面的岩藻聚糖使铜绿假单胞菌的黏附率降低了约 90%。此外，AFM 观察表明，多糖还抑制了细菌在表面上的扩散，但不抑制细胞间的相互作用。最后，在非运动生物表皮葡萄球菌上测试了表面抗黏附效果的持久性。结果表明，这种效果至少可以维持 24 小时。
