Ankara University Faculty of Pharmacy, Pharmacognosy Department, 06100, Tandogan, Çankaya, Ankara, Turkey.
Gazi University Faculty of Pharmacy, Pharmacognosy Department, 06330, Etiler, Yenimahalle, Ankara, Turkey.
Biomed Pharmacother. 2017 Jul;91:1096-1105. doi: 10.1016/j.biopha.2017.05.040. Epub 2017 May 16.
Ononis species are used for their laxative, diuretic, analgesic, anti-inflammatory, antiviral, cytotoxic and antifungal effects as well as against skin diseases for wound healing activity. In the light of this information n-hexane, ethylacetate and methanol extracts prepared from Ononis spinosa L. subsp. leiosperma (Boiss.) Sirj., Ononis variegata L., Ononis viscosa L. subsp. brevifolia (DC) Nym. and Ononis natrix L. subsp. natrix L. were tested for their wound healing, anti-inflammatory and antioxidant activities. Linear incision and circular excision wound models and hydroxypyroline estimation assay were used for the wound healing activity. For the assessment of chronic inflammation FCA-induced arthritis and for acute inflammation carrageenan-induced hind paw edema, TPA-induced ear edema and acetic acid-induced increase in capillary permeability tests were conducted. 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) scavenging activity assay, reducing power assay and hydroxyl radical (OH) scavenging assay were used for determining antioxidant activities of the extracts. Results showed that O. spinosa subsp. leiosperma roots ethyl acetate extract exhibited remarkable wound healing activity with the 42.6% tensile strength value on the linear incision wound model and 60.1% reduction of the wound area at the day 12 on the circular excision wound model. Hydroxyproline content of the tissue treated by O. spinosa subsp. leiosperma roots ethyl acetate extract was found to be 41.3μg/mg. Acetic acid induced increase in capillary permeability test results revealed that O. spinosa subsp. leiosperma roots ethyl acetate extract and O. spinosa subsp. leiosperma roots methanol extract inhibited inflammation by 40.4% and 35.4% values respectively. O. spinosa subsp. leiosperma roots ethyl acetate extract showed 21.2-27.2% inhibition in carrageenan-induced hind paw edema test while did not posses activity on TPA-induced ear edema and FCA-induced arthritis models.
金雀花属植物因其具有通便、利尿、镇痛、抗炎、抗病毒、细胞毒性和抗真菌作用，以及对皮肤病具有愈合活性而被使用。鉴于此信息，从金雀花属植物种的 Spinosa L. 亚种 leiosperma（Boiss.）Sirj.、variegata L.、viscosa L. 亚种 brevifolia（DC）Nym. 和 natrix L. subsp. natrix L. 中制备的正己烷、乙酸乙酯和甲醇提取物被测试了其愈合伤口、抗炎和抗氧化活性。线性切口和圆形切除伤口模型和羟脯氨酸估计测定法用于评估伤口愈合活性。为了评估慢性炎症 FCA 诱导的关节炎和急性炎症角叉菜胶诱导的后爪水肿、TPA 诱导的耳水肿和醋酸诱导的毛细血管通透性增加，进行了试验。2,2-二苯基-1-苦基肼基（DPPH）自由基清除测定法、2,2-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）清除活性测定法、还原能力测定法和羟基自由基（OH）清除测定法用于测定提取物的抗氧化活性。结果表明，金雀花属植物 Spinosa L. 亚种 leiosperma 根的乙酸乙酯提取物在线性切口伤口模型中具有显著的愈合活性，其拉伸强度值为 42.6％，在圆形切除伤口模型中第 12 天的伤口面积减少了 60.1％。用金雀花属植物 Spinosa L. 亚种 leiosperma 根的乙酸乙酯提取物处理的组织中的羟脯氨酸含量为 41.3μg/mg。醋酸诱导的毛细血管通透性增加试验结果表明，金雀花属植物 Spinosa L. 亚种 leiosperma 根的乙酸乙酯提取物和金雀花属植物 Spinosa L. 亚种 leiosperma 根的甲醇提取物分别抑制了炎症的 40.4％和 35.4％。金雀花属植物 Spinosa L. 亚种 leiosperma 根的乙酸乙酯提取物在角叉菜胶诱导的后爪水肿试验中显示出 21.2-27.2％的抑制作用，而对 TPA 诱导的耳水肿和 FCA 诱导的关节炎模型没有活性。
